High-loading ficin@AuNPs on polymer-UiO-66 surface with enhanced peroxidase-mimetic catalytic activity for colourimetric detection of dopamine
Related Experiment Video
Updated: Jun 12, 2025
Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection
Published on: May 13, 2019
Yutong Liu1,2, Lin Tian1,2, Zhenwen Zhao1,3
1Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Beijing National Laboratory for Molecular Sciences, Chinese Academy of Sciences, Beijing, 100190, P. R. of China.
Abstract:
Recently, MOFs@AuNPs composites-based catalysts via anchoring of AuNPs onto metal-organic-frameworks (MOFs) have attracted great attention. However, the influence of the AuNPs loading amounts on the catalytic activity of MOFs@AuNPs composites remains largely unexplored. Here, ficin (Fic) protected AuNPs (Fic@AuNPs) anchored onto the surface of UiO-66-NH2 (UiO) modified with poly(2-vinyl-4,4-dimethyl-2-oxazolidine) (PV) were designed and constructed. The UiOPVFic@AuNPs composites with longer PV chains leading to high-loading Fic@AuNPs exhibited intense peroxidase (POD)-mimetic activity in 3,3'5,5'-tetramethylbenzidine (TMB) oxidation. Further, following the colour-fading, dopamine (DA) was sensitively and selectively monitored in the composites-TMB-H2O2 system. The portable smartphone sensing platform-based colourimetric method had good linearity ranging from 3.34 to 36.7 μM (R2 = 0.995), with a limit of detection of 0.3 μM. This protocol explores high-loading AuNPs on polymer-MOFs composites, providing deep insights into understanding catalytic activity improvements of polymer-MOFs@AuNPs catalysts and revealing their application potential in real biological samples analysis.
