Purification and identification of Streptomyces aureofaciens lD13 antibiotic

Complete extraction of S. aureofaciens lD13 antibiotic was achieved by adding n-butanol to the clarified culture filtrate (v/2v) at pH 8.0. Using the column chromatography technique, 85.7% of the initial amount of the antibiotic was obtained in a purified form. Data of the Rf values of the antibiotic in different organic solvents revealed that it belongs to the tetracycline group. The antibiotic was chromatographically analyzed, using the thin-layer technique. UV and IR spectra, optical rotation, melting point as well as 15 colour reactions were also determined.