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Updated: Jun 12, 2025
Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
Published on: February 12, 2019
Leukocyte Extracellular Vesicles Predict Progression of Systolic Dysfunction in Heart Failure with Mildly Reduced
Aleksandra Gąsecka1,2, Aleksander Siniarski3,4, Piotr Duchnowski5
11St Chair and Department of Cardiology, Medical University of Warsaw, Banacha 1a, 02-097, Warsaw, Poland.
Insights
Higher concentrations of leukocyte extracellular vesicles (EVs) predict systolic dysfunction progression in heart failure with mildly-reduced ejection fraction (HFmrEF). This finding aids risk stratification for HFmrEF patients.
Area of Science:
- Cardiology
- Biomarkers
- Heart Failure Research
Background:
- Risk stratification for heart failure with mildly-reduced ejection fraction (HFmrEF) is complex.
- Identifying reliable biomarkers for disease progression is crucial for effective management.
Purpose of the Study:
- To evaluate advanced glycation end products (AGEs) and extracellular vesicles (EVs) as predictors of systolic and diastolic dysfunction progression in HFmrEF.
- To determine if leukocyte EVs can identify patients at higher risk for HFmrEF progression.
Main Methods:
- Assessed skin AGE accumulation using AGE Reader.
- Measured plasma EV concentrations via flow cytometry in 74 HFmrEF patients.
- Monitored systolic and diastolic dysfunction progression over a 6.5-month follow-up period.
Main Results:
- Leukocyte EV concentrations were significantly higher in patients with systolic dysfunction progression (p=0.002).
- Leukocyte EVs predicted systolic dysfunction progression with 75.0% sensitivity and 58.3% specificity.
- Increased leukocyte EVs were associated with a 4.7-fold higher odds of systolic dysfunction progression, independent of other clinical factors.
Conclusions:
- Elevated leukocyte EV concentrations are a significant predictor of systolic dysfunction progression in HFmrEF.
- Skin AGE levels and other EV subtypes did not show a significant association with dysfunction progression.
- Leukocyte EVs represent a promising biomarker for risk stratification in HFmrEF.
Abstract:
Risk stratification in heart failure with mildly-reduced ejection fraction (HFmrEF) remains challenging. We evaluated the predictive value of advanced glycation end products (AGEs) and plasma concentrations of extracellular vesicles (EVs) for the systolic and diastolic dysfunction progression in HFmrEF patients. Skin AGE accumulation was measured using AGE Reader. Plasma EV concentrations were measured using flow cytometry. Among 74 patients enrolled, 13 (18%) had systolic dysfunction progression and 5 (7%) had diastolic dysfunction progression during 6.5 months follow-up. Leukocyte EVs concentrations were higher in patients with systolic dysfunction progression (p = 0.002) and predicted the progression with 75.0% sensitivity and 58.3% specificity, independent of other clinical variables (OR 4.72, 95% CI 0.99-22.31). Skin AGE levels and concentrations of other EV subtypes were not associated with systolic or diastolic dysfunction progression. Increased leukocyte EVs concentrations are associated with 4.7-fold higher odds of systolic dysfunction progression in HFmrEF patients.
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