Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cleavage and Blastulation01:33

Cleavage and Blastulation

44.8K
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
44.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CRH expression and function in mouse uterus during early pregnancy.

Reproduction, fertility, and development·2026
Same author

Differential Expression and Function of Arginase in Mouse Uterus During Early Pregnancy.

International journal of molecular sciences·2026
Same author

Correction: Embryo-derive TNF promotes decidualization via fibroblast activation.

eLife·2026
Same author

Effect of Diethylstilbestrol on Implantation and Decidualization in Mice.

International journal of molecular sciences·2025
Same author

Effects of Acrylamide on Mouse Implantation and Decidualization.

International journal of molecular sciences·2025
Same author

Primary cilia prevent activation of the cGAS-STING pathway during mouse decidualization.

Communications biology·2025

Related Experiment Video

Updated: Jun 12, 2025

Author Spotlight: Advancing Therapeutic Strategies for Improving Pregnancy Rates by Analyzing Embryo-Endometrium Interactions
05:13

Author Spotlight: Advancing Therapeutic Strategies for Improving Pregnancy Rates by Analyzing Embryo-Endometrium Interactions

Published on: June 21, 2024

768

Embryo-Derived Cathepsin B Promotes Implantation and Decidualization by Activating Pyroptosis.

Meng-Yuan Li1,2, Ying Wu2, Hao-Lan Tang1

  • 1Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountain Region, College of Animal Science, Guizhou University, Guiyang, 550025, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|September 24, 2024
PubMed
Summary

Modest pyroptosis, a form of cell death, is essential for embryo implantation and decidualization. However, excessive pyroptosis can lead to pregnancy failure, highlighting a critical balance during early pregnancy.

Keywords:
implantationinflammatorypyroptosis

More Related Videos

Generation of a Mouse Artificial Decidualization Model with Ovariectomy for Endometrial Decidualization Research
06:38

Generation of a Mouse Artificial Decidualization Model with Ovariectomy for Endometrial Decidualization Research

Published on: July 27, 2022

5.2K
Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization
14:55

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

Published on: September 1, 2018

10.2K

Related Experiment Videos

Last Updated: Jun 12, 2025

Author Spotlight: Advancing Therapeutic Strategies for Improving Pregnancy Rates by Analyzing Embryo-Endometrium Interactions
05:13

Author Spotlight: Advancing Therapeutic Strategies for Improving Pregnancy Rates by Analyzing Embryo-Endometrium Interactions

Published on: June 21, 2024

768
Generation of a Mouse Artificial Decidualization Model with Ovariectomy for Endometrial Decidualization Research
06:38

Generation of a Mouse Artificial Decidualization Model with Ovariectomy for Endometrial Decidualization Research

Published on: July 27, 2022

5.2K
Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization
14:55

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

Published on: September 1, 2018

10.2K

Area of Science:

  • Reproductive biology
  • Cellular and molecular biology
  • Immunology

Background:

  • Embryo implantation and decidualization are critical for pregnancy success.
  • The regulation of inflammatory responses during these processes remains unclear.
  • Pyroptosis, a programmed inflammatory cell death, is mediated by gasdermin D (GSDMD).

Purpose of the Study:

  • To investigate the role of pyroptosis in embryo implantation and decidualization.
  • To elucidate the mechanisms by which blastocysts regulate pyroptosis.
  • To determine the clinical relevance of pyroptosis in human implantation disorders.

Main Methods:

  • In vivo studies in pseudopregnant and pregnant mice.
  • In vitro experiments using cultured epithelial cells and organoids.
  • Analysis of pyroptosis-related protein levels and inflammatory markers.

Main Results:

  • Pyroptosis occurs in epithelial cells at the implantation site during early pregnancy.
  • Blastocyst-derived cathepsin B (CTSB) induces pyroptosis, leading to IL-18 secretion.
  • IL-18 activates ADAM12, processing epiregulin (EREG), which enhances decidualization.
  • Elevated pyroptosis markers are found in human endometrium with recurrent implantation failure.
  • Lipopolysaccharide-induced excessive pyroptosis causes implantation failure in mice.

Conclusions:

  • A modest level of pyroptosis is beneficial for successful embryo implantation and decidualization.
  • Dysregulated or excessive pyroptosis can impair pregnancy and lead to implantation failure.
  • Pyroptosis plays a dual role in reproductive success, with implications for treating implantation disorders.