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Biomechanics Model to Characterize Atomic Force Microscopy-Based Virus-Host Cell Adhesion Measurements
Jiajun Wang1, Matthew Ziarnik1, X Frank Zhang2
1Department of Bioengineering, Lehigh University, Bethlehem, Pennsylvania 18015, United States.
The Journal of Physical Chemistry. B
|September 24, 2024
Summary
We developed a new model to quantify virus-cell adhesion using atomic force microscopy (AFM). This model accurately predicts pull-off forces, offering a refined understanding of viral interactions and improving AFM data interpretation.
Area of Science:
- Biophysics
- Materials Science
- Virology
Background:
- Virus-cell adhesion is crucial for infection.
- Existing models lack precision in quantifying adhesive properties.
- Atomic force microscopy (AFM) provides force-distance data but requires robust models for interpretation.
Purpose of the Study:
- To present a refined cohesive zone model for virus-cell adhesion.
- To enable quantitative extraction of adhesive properties from AFM measurements.
- To account for receptor deformability in viral adhesion.
Main Methods:
- Extended a continuum model using a cohesive zone model with pull-off stress and characteristic displacement.
- Represented viral receptors as a Winkler foundation.
- Compared model-simulated force-separation curves with experimental AFM data.
Main Results:
- The model effectively explains AFM pull-off force traces.
- Successfully quantified adhesion parameters, including pull-off stress and displacement.
- Demonstrated the model's ability to account for receptor deformability.
Conclusions:
- The refined model offers a more accurate understanding of virus-cell adhesion.
- Provides a framework for interpreting and predicting AFM force spectroscopy measurements.
- Facilitates quantitative analysis of adhesive interactions involving viral proteins and cell surface components.
