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Ctr9 promotes virulence of Candida albicans by regulating methionine metabolism

Jiyeon Park1, Shinae Park1, Jueun Kim1

  • 1Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University, Chuncheon, Republic of Korea.

Virulence
|September 24, 2024
PubMed

Insights

The Paf1 complex (Paf1C) regulates metabolic pathways in Candida albicans. Deleting CTR9, a Paf1C component, reduces fungal virulence by impairing methionine metabolism and cell proliferation.

Area of Science:

  • Mycology
  • Molecular Biology
  • Pathogenesis

Background:

  • Candida albicans is an opportunistic fungal pathogen.
  • Its pathogenicity is linked to metabolic pathway regulation.
  • The Paf1 complex (Paf1C) is a conserved transcriptional regulator.

Purpose of the Study:

  • To investigate the role of Paf1C in C. albicans metabolic pathways.
  • To determine how Paf1C influences C. albicans pathogenicity.

Main Methods:

  • Generated Paf1C knockout mutant strains using CRISPR-Cas9.
  • Assessed pathogenicity via macrophage interaction and mouse survival assays.
  • Analyzed growth patterns and performed transcriptome analysis under various conditions.

Main Results:

  • CTR9 deletion attenuated C. albicans virulence in vitro and in vivo.
  • Reduced virulence correlated with decreased cell abundance and impaired metabolic processes.
  • CTR9 deletion downregulated methionine biosynthesis and essential hyphal genes.

Conclusions:

  • Ctr9 plays a vital role in C. albicans pathogenicity.
  • Methionine metabolism regulated by Ctr9 is crucial for virulence.
  • Paf1C is a key regulator of C. albicans virulence and metabolic processes.
Keywords:
Candida albicansCtr9Methionine metabolismPaf1 complexPathogenicity

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