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Updated: Jun 12, 2025
A Polyaniline-based Sensor of Nucleic Acids
Published on: November 1, 2016
A tunable LDI-MS platform assisted by metal-phenolic network-coated AuNPs for sensitive and customized detection of
Tong Hu1, Qi Sang1, Dingyitai Liang1
1State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering and Institute of Medical Robotics, Division of Cardiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200030, PR China; Shanghai Jiao Tong University Sichuan Research Institute, Chengdu, 610213, PR China.
Abstract:
This study introduces a novel approach for the sensitive and accurate detection of small molecule metabolites, employing metal-phenolic network (MPN) functionalized AuNPs as both adsorbent and matrix to enhance laser desorption/ionization mass spectrometry (LDI-MS) performance. The MPN comprising tannic acid (TA) and transition metal ions (Fe3+, Co2+, Ni2+, Cu2+, or Zn2+) was coated on the surface of AuNPs, forming metal-TA network-coated AuNPs (M-TA@AuNPs). The M-TA@AuNPs provided a tunable surface for specific interactions with analytes, displaying distinct enrichment efficacies for different amino acids, especially for Cu-TA@AuNPs exhibiting the highest affinity for histidine (His). Under the optimized condition, the proposed method enabled ultrasensitive detection of His, with good linearity (R2 = 0.9627) in the low-concentration range (50 nM-1 μM) and a limit of detection (LOD) as low as 0.9 nM. Furthermore, the method was successfully applied to detect His from human urine samples, showcasing its practical applications in clinical diagnostics, particularly in the realm of amino acid-based targeted metabolomics.
