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Updated: Jun 12, 2025
Flow Cytometric Characterization of Murine B Cell Development
Published on: January 22, 2021
Profiling of B cells and their subsets by whole blood gene expression analysis versus flow cytometry in multiple
Sahla El Mahdaoui1, Marina Rode von Essen1, Marie Mathilde Hansen1
1Danish Multiple Sclerosis Center, Department of Neurology, Copenhagen University Hospital, Rigshospitalet, Glostrup, Denmark.
Abstract:
We investigated if differentially expressed mRNA targets could be used as surrogate markers for circulating B cells and subsets. In paired blood samples from patients with untreated, anti-CD20-treated, fingolimod-treated, and natalizumab-treated multiple sclerosis, whole blood expression of CD19 correlated with B cell counts determined by flow cytometry, ROR1 with transitional B cells, TCL1A and ZNF727 with naïve B cells, NEXMIF with memory B cells and BCMA with plasmablasts. CD19 expression distinguished patients with B cell repletion and may be used as an alternative to flow cytometry, but NEXMIF was unsuitable for memory B cell monitoring in rituximab-treated patients.
