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Corn stover variability drives differences in bisabolene production by engineered Rhodotorula toruloides.

Onyinye Okonkwo1, Chang Dou1, Ethan Oksen1

  • 1Advanced Biofuels and Bioproducts Process Development Unit, Lawrence Berkeley National Lab, Berkeley, CA 94608, USA.

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|September 24, 2024
PubMed
Summary
This summary is machine-generated.

Understanding corn stover variability is key for efficient bisabolene production. Feedstock differences impact microbial growth and product yield, with the stalk fraction yielding the most bisabolene (8.89 g/L).

Keywords:
R. toruloidesAnatomic fractionsBisaboleneCorn stover variabilityFermentationSelf-heating

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Area of Science:

  • Biotechnology
  • Microbiology
  • Biochemical Engineering

Background:

  • Microbial conversion of lignocellulosic biomass offers a sustainable route for biofuels.
  • Rhodotorula toruloides is engineered for bisabolene production, a sustainable aviation fuel precursor.
  • Feedstock heterogeneity significantly impacts bioconversion efficiency, but is understudied.

Purpose of the Study:

  • To investigate how variations in corn stover batches, anatomical fractions, and storage conditions affect bisabolene production by R. toruloides.
  • To understand the influence of feedstock heterogeneity on microbial growth and product titer.
  • To correlate protein expression changes with feedstock variations.

Main Methods:

  • Corn stover feedstocks underwent deacetylation, mechanical refining, and enzymatic hydrolysis.
  • Engineered R. toruloides fermented hydrolysates to produce bisabolene.
  • Global protein expression analysis (proteomics) was used to compare microbial responses.

Main Results:

  • Bisabolene production varied significantly based on corn stover source and fraction.
  • The stalk fraction of corn stover hydrolysate yielded the maximum bisabolene (8.89 ± 0.47 g/L).
  • Proteomics revealed significant upregulation/downregulation of proteins involved in carbohydrate metabolism, energy production, and ion transport.

Conclusions:

  • Feedstock heterogeneity presents complex challenges and opportunities for bisabolene production.
  • R. toruloides exhibits coordinated metabolic responses to feedstock variations, such as iron-sulfur cluster protein regulation.
  • Optimizing feedstock selection and pretreatment is crucial for enhancing bisabolene yield and bioprocess economics.