Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chronic neuroinflammation after acute SARS-Cov-2 infection induces retinal damage in the hACE2 transgenic mouse model.

Journal of neuroimmunology·2026
Same author

Thin-Film Eddy Current Sensors for Proximity Detection in Smart Capillary Pick-and-Place.

ACS sensors·2026
Same author

Associations of Circadian Rhythms with Cognitive Performance in Patients with Amnestic Mild Cognitive Impairment (aMCI).

Journal of clinical medicine·2026
Same author

A Randomized Multicenter Study Comparing Low-Viscosity with Comparator 0.3% Hyaluronic Acid for the Treatment of Dry Eye Disease.

Ophthalmology and therapy·2026
Same author

Elevated CD45<sup>+</sup>CD11c<sup>+</sup>CX3CR1<sup>+</sup> Cells in Optic Nerves and Their Relation to Optic Nerve Function in an Experimental Autoimmune Encephalomyelitis Model.

The American journal of pathology·2026
Same author

Surface-modified anode current collectors <i>via</i> lithiophilic zinc-based bimetallic co-electrodeposition for lithium-metal batteries.

Nanoscale horizons·2026

Related Experiment Video

Updated: Jun 12, 2025

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
09:30

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Published on: May 24, 2019

7.4K

Label-Free Exosome Analysis by Surface-Enhanced Raman Scattering Spectroscopy with Laser-Ablated Silver Nanoparticle

Jong-Eun Park1, Hyeono Nam2, June Sik Hwang3

  • 1Department of Mechanical Engineering, The State University of New York, Korea (SUNY Korea), Incheon, 21985, Republic of Korea.

Advanced Healthcare Materials
|September 25, 2024
PubMed
Summary
This summary is machine-generated.

Early breast cancer detection is vital. This study introduces a rapid, label-free method using enhanced Raman scattering (SERS) with silver nanoparticles for exosome detection, improving point-of-care diagnostics.

Keywords:
breast cancerexosomesnanofabricationpoint‐of‐care testing (POCT)rapid and label‐free detectionselective laser ablation and melting (SLAM)surface‐enhanced raman scattering (SERS) spectroscopy

More Related Videos

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.3K
Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

1.5K

Related Experiment Videos

Last Updated: Jun 12, 2025

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
09:30

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Published on: May 24, 2019

7.4K
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.3K
Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

1.5K

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Spectroscopy

Background:

  • Early breast cancer diagnostics are critical for preventing metastasis and relapse.
  • Exosomes in liquid biopsies offer potential for early detection but face sensitivity and diagnostic tool limitations.
  • Surface-enhanced Raman Scattering (SERS) shows promise for exosome detection, yet SERS probe fabrication is inefficient for large-scale production.

Purpose of the Study:

  • To develop a rapid, label-free method for detecting breast cancer-derived exosomes.
  • To create an efficient, large-scale production-compatible SERS substrate.
  • To evaluate the feasibility of point-of-care testing (POCT) for breast cancer exosomes.

Main Methods:

  • Fabrication of silver-nanoparticle-based SERS substrates using selective laser ablation and melting (SLAM) with silver nanowires.
  • Optimization of laser process parameters for rapid, low-energy SERS substrate production.
  • Evaluation of SERS substrate performance (sensitivity, reproducibility, stability, renewability) using rhodamine 6G and statistical analysis of SERS spectra from breast cancer exosomes.

Main Results:

  • Rapid and low-energy fabrication of SERS substrates was achieved by optimizing SLAM parameters with silver nanowires.
  • The SERS substrate demonstrated excellent sensitivity, reproducibility, stability, and renewability.
  • Statistical analysis of SERS spectra successfully distinguished exosomes from malignant breast cancer cells and non-tumorigenic cells, showing POCT feasibility.

Conclusions:

  • The proposed SLAM-fabricated SERS substrate enables rapid, label-free detection of breast cancer exosomes.
  • This approach overcomes limitations in current exosome detection methods, paving the way for improved POCT.
  • The cost-effective and scalable fabrication method holds potential for broader biomedical applications.