A qualitative agar gel immunoprecipitin (IP) test for detection of fecal occult human hemoglobin
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Abstract:
A versatile, qualitative, agarose gel immunoprecipitation (IP) test for the detection of fecal occult hemoglobin (Hb) was developed to provide a more accurate method for the detection of occult blood in stool. This test allowed detection of 0.2 mg human hemoglobin/g of stool after a 2-h incubation. In addition, a specimen application device which can accommodate a variety of specimens was developed for this test. A study of 252 clinical stool specimens revealed a close correlation in the results obtained at three separate laboratories. The IP test identified blood in 11 out of 24 specimens collected from patients with colorectal carcinoma as compared to 4 of 24 specimens positive with a guaiac-based Hemoccult II test. The simplicity of immunoprecipitation coupled with the high sensitivity and specificity of this technique suggests that this new test would be a very useful and effective means with which to screen for occult blood in stool.
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