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Updated: Jun 12, 2025

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
Published on: July 5, 2018
Strategy for Construction of Homogeneous Glycoproteins in Mammalian Cells
Chang-Hee Lee1, Hui Li1, Ji Young Hyun2,3
1Department of Chemistry, Yonsei University, 03722 Seoul, Republic of Korea.
Abstract:
A general strategy that combines genetic code expansion with bio-orthogonal ligation techniques was developed and utilized to prepare homogeneously glycosylated receptors on the surface of mammalian cells. Using this approach, conjugates of the cell-surface oxytocin receptor (OTR) with oligosaccharides were efficiently generated in the cells. Cell studies revealed that glycans linked to the OTR are not essential for agonist-induced calcium flux and its internalization into cells via an OTR-mediated endocytosis.
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