Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

1.6K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
1.6K
GPCR Desensitization01:12

GPCR Desensitization

5.9K
G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
5.9K
Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

1.4K
Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
1.4K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

2.7K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.7K
Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists01:28

Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists

153
Neurokinin 1 (NK1) receptors are distributed across the GI tract, vagal afferents, and key CNS regions including the central vomiting center and chemoreceptor trigger zone (CTZ) Chemotherapy agents stimulate enterochromaffin cells in the gastrointestinal (GI) tract to release large amounts of substance P (SP). SP is a neuropeptide released by specific sensory nerves in response to many different stressors, including those in the GI mucosa affected by chemotherapy.  SP binds and activates...
153

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Baseline radon concentrations in uranium-rich and faulted zones of the Eastern Cordillera, Central Andes, Peru.

Journal of environmental radioactivity·2026
Same author

CAR-NK Cells in B-cell Lymphoma: A New Frontier toward Accessible and Scalable Cellular Therapy.

Blood cancer discovery·2026
Same author

[Emergency Aortic Care at a Newly Established University Aortic Centre - Outcome Treatment Comparison of Acute Thoracic/Thoracoabdominal Pathologies to an Earlier Cohort at the Same Site].

Zentralblatt fur Chirurgie·2026
Same author

A genetic set-point for natural killer cells.

Nature immunology·2026
Same author

Taxonomical modeling and classification in space hardware failure reporting.

Scientific reports·2026
Same author

Preclinical assessment of MAGE-A4-specific TCR-NK cells against solid tumors.

Immunotherapy advances·2026

Related Experiment Video

Updated: Jun 12, 2025

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells
06:55

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells

Published on: February 22, 2020

18.5K

The G Protein-Coupled Receptor GPR56 Is an Inhibitory Checkpoint for NK Cell Migration.

Daniel Palacios1,2, Rakesh Kumar Majhi1,3, Edina K Szabo1,2

  • 1Department of Cancer Immunology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway.

Journal of Immunology (Baltimore, Md. : 1950)
|September 25, 2024
PubMed
Summary
This summary is machine-generated.

G protein-coupled receptor 56 (GPR56) acts as a checkpoint for natural killer (NK) cell migration. Inhibiting GPR56 enhances NK cell movement, offering potential strategies to improve cancer immunotherapy.

More Related Videos

Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons
09:40

Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons

Published on: June 30, 2022

2.1K
Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
12:52

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

15.8K

Related Experiment Videos

Last Updated: Jun 12, 2025

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells
06:55

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells

Published on: February 22, 2020

18.5K
Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons
09:40

Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons

Published on: June 30, 2022

2.1K
Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
12:52

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

15.8K

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • G protein-coupled receptors (GPCRs) are crucial for physiological functions, including cell migration.
  • GPR56 (ADGRG1) is an adhesion GPCR expressed on various cell types, including lymphocytes like NK cells.

Purpose of the Study:

  • To investigate the role of GPR56 in the migration of human NK cell subsets.
  • To explore the potential of targeting GPR56 for enhancing NK cell infiltration in cancer.

Main Methods:

  • RNA-sequencing and high-resolution flow cytometry to analyze GPR56 expression during NK cell differentiation.
  • Small interfering RNA (siRNA) to silence GPR56 and assess its impact on NK cell migration.
  • Agonistic stimulation of GPR56 to observe receptor internalization and deactivation.

Main Results:

  • GPR56 expression increases with NK cell differentiation, peaking in adaptive NK cells.
  • GPR56 silencing enhances spontaneous and chemokine-induced NK cell migration.
  • GPR56 ligation leads to receptor internalization, nuclear translocation of transcriptional coactivator with PDZ-binding motif, and increased actin polymerization.

Conclusions:

  • GPR56 functions as an upstream checkpoint regulating the migration of highly differentiated NK cells.
  • Modulating GPR56 activity could potentially improve NK cell infiltration into tumor tissues for cancer immunotherapy.