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Oocytes Vitrification Using Automated Equipment Based on Microfluidic Chip.

Jing Shen1,2,3, Zixuan Yu1, Weijie Li1,2,3

  • 1Institute of Biothermal Science & Technology, University of Shanghai for Science and Technology, Shanghai, 200093, China.

Annals of Biomedical Engineering
|September 25, 2024
PubMed
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A new microfluidic-based automated vitrification (MAV) device significantly improves oocyte survival and developmental rates compared to manual methods. Optimized CPA delivery protocols within the MAV system further enhance vitrification success for fertility preservation.

Area of Science:

  • Assisted Reproductive Technology
  • Cryobiology
  • Microfluidics

Background:

  • Oocyte vitrification is crucial for assisted reproduction and fertility preservation.
  • Manual cryoprotectant agent (CPA) manipulation risks osmotic shock and requires expert skill.
  • Automated systems are needed to standardize and improve vitrification outcomes.

Purpose of the Study:

  • To develop and evaluate a microfluidic-based automated vitrification (MAV) device for oocyte cryopreservation.
  • To compare the efficacy of MAV against the manual Cryotop/QC method.
  • To optimize CPA loading and removal protocols within the MAV system.

Main Methods:

  • A microfluidic system was engineered for precise, linear CPA concentration adjustment.
  • The MAV device integrated CPA handling with the vitrification process for automation.
Keywords:
Automated vitrificationMicrofluidicsOocyteOsmotic injury

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  • Oocytes were vitrified using MAV with varying CPA delivery segments (1, 2, and 4) and compared to manual Cryotop/QC.

    • Main Results:

    • MAV method yielded significantly higher oocyte survival (80.44%), cleavage (54.17%), and blastocyst rates (32.95%) than manual methods (73.35%, 43.73%, 23.67%).
    • Oocytes vitrified using the 4-segment CPA delivery protocol showed superior outcomes: 86.18% survival, 63.29% cleavage, and 45.58% blastocyst rates.
    • Specific CPA concentration protocols (VS1-TS1) demonstrated the highest rewarming survival, while others (VS3-TS3) showed a higher blastocyst rate post-activation.

    Conclusions:

    • The MAV device offers a promising automated solution for oocyte vitrification, improving success rates.
    • Optimized multi-segment CPA delivery protocols enhance oocyte cryopreservation outcomes.
    • This technology has significant potential for advancing fertility preservation and assisted reproduction.