Related Experiment Video

Updated: Jun 12, 2025

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
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Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

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Unveiling the DHX15-G-patch interplay in retroviral RNA packaging

Alžběta Dostálková1, Ivana Křížová1, Petra Junková2,3

  • 1Department of Biotechnology, University of Chemistry and Technology, 166 28 Prague, Czech Republic.

Proceedings of the National Academy of Sciences of the United States of America
|September 25, 2024
PubMed

Insights

Mason-Pfizer monkey virus (M-PMV) hijacks the cellular RNA helicase DHX15 for replication. This virus recruits DHX15 to the cytoplasm, essential for viral genome packaging and infectivity.

Area of Science:

  • Virology
  • Molecular Biology
  • Cellular Biology

Background:

  • Viruses often manipulate host cell machinery for replication.
  • Cellular RNA helicases play crucial roles in RNA processing and regulation.

Purpose of the Study:

  • To investigate the role of the RNA helicase DHX15 in Mason-Pfizer monkey virus (M-PMV) replication.
  • To elucidate the mechanism by which M-PMV interacts with and utilizes DHX15.

Main Methods:

  • Advanced genetic engineering techniques were employed.
  • Cellular localization of DHX15 was tracked using advanced microscopy.
  • Viral genome packaging efficiency and infectivity were assessed.

Main Results:

  • M-PMV recruits the cellular RNA helicase DHX15 from the nucleus to the cytoplasm.
  • This recruitment mimics cellular mechanisms and is essential for viral assembly.
  • DHX15 interaction is critical for proper viral genome packaging and M-PMV infectivity.

Conclusions:

  • M-PMV employs a sophisticated strategy to hijack host DHX15 for viral replication.
  • This interaction provides insights into viral manipulation of host RNA processing pathways.
  • Findings suggest potential therapeutic targets for controlling M-PMV and related viruses.
Keywords:
DEAH-box RNA helicaseDHX15G-patchgRNA packagingretrovirus

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