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Related Experiment Video

Updated: Jun 12, 2025

Fluorescence Lifetime Macro Imager for Biomedical Applications
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Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

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Light-field tomographic fluorescence lifetime imaging microscopy.

Yayao Ma1, Jongchan Park1, Luzhe Huang1,2,3

  • 1Department of Bioengineering, University of California, Los Angeles, CA 90095.

Proceedings of the National Academy of Sciences of the United States of America
|September 25, 2024
PubMed
Summary
This summary is machine-generated.

We developed light-field tomographic FLIM (LIFT-FLIM), a computational imaging technique that significantly reduces scanning steps for faster, high-resolution 3D fluorescence lifetime imaging microscopy. This breakthrough enhances molecular-level biological visualization.

Keywords:
3D imagingfluorescence lifetime imaging microscopylight field imaging

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Area of Science:

  • Biophotonics and Imaging Science
  • Molecular and Cellular Biology
  • Computational Imaging

Background:

  • Fluorescence lifetime imaging microscopy (FLIM) visualizes biological samples at the molecular level by measuring fluorescence decay rates.
  • Conventional FLIM faces challenges with long acquisition times and extensive scanning, especially for 3D imaging.
  • High-resolution lifetime maps are crucial for understanding molecular interactions and cellular environments.

Purpose of the Study:

  • To develop a computational imaging technique to overcome the limitations of conventional FLIM systems.
  • To enable faster, more data-efficient acquisition of volumetric fluorescence lifetime images.
  • To reduce the scanning requirements for high-resolution 3D FLIM.

Main Methods:

  • Introduced light-field tomographic FLIM (LIFT-FLIM), a novel computational imaging approach.
  • Utilized low-dimensional detectors with high temporal bandwidth for efficient data acquisition.
  • Demonstrated LIFT-FLIM using a linear single-photon avalanche diode array.

Main Results:

  • LIFT-FLIM significantly reduces scanning steps compared to traditional FLIM methods.
  • Achieved highly data-efficient acquisition of volumetric fluorescence lifetime images.
  • Showcased unparalleled single-photon detection sensitivity and expanded functionality to spectral FLIM.
  • Applied LIFT-FLIM to high-content multiplexed imaging of lung organoids.

Conclusions:

  • LIFT-FLIM offers a powerful solution for rapid, high-resolution 3D fluorescence lifetime imaging.
  • The technique enhances molecular-level visualization in biological systems.
  • LIFT-FLIM holds significant potential for advancing both basic and translational biomedical research.