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Updated: Jun 12, 2025

Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
Protocol for engineering binding domains to recognize ligand-bound receptors by using yeast surface display
Markus Dobersberger1, Delia Sumesgutner2, Charlotte U Zajc2
1Department of Chemistry, Institute of Biochemistry, BOKU University, Vienna 1190, Austria.
Abstract:
Yeast surface display is a versatile protein engineering technology, enabling precise control of the applied selection pressure. We present a yeast-surface-display-based protocol for the enrichment of binders specifically recognizing ligand-bound receptors. We describe steps for magnetic bead selections, random mutagenesis, and flow cytometric sorting, followed by library sequencing and detailed analysis of enriched clones. While this approach is exemplified with rcSso7d-based libraries and epidermal growth factor (EGF)-epidermal growth factor receptor (EGFR) complexes, it can also be adapted to other binder scaffolds and ligand-receptor systems. For complete details on the use and execution of this protocol, please refer to Dobersberger et al.1.
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