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OptoAssay-Light-controlled dynamic bioassay using optogenetic switches
Nadine Urban1,2,3, Maximillian Hörner3, Wilfried Weber3,4,5
1University of Freiburg, FIT Freiburg Center for Interactive Materials and Bioinspired Technologies, 79110 Freiburg, Germany.
Science Advances
|September 25, 2024
Summary
We developed OptoAssay, a novel light-controlled diagnostic assay. This wash- and pump-free technology offers sensitive detection and enables point-of-care diagnostics, even in resource-limited settings.
Area of Science:
- Biotechnology
- Assay Development
- Point-of-Care Diagnostics
Background:
- Current bioassays face limitations in complex workflows, requiring multiple steps like washing and external fluidic control.
- Optogenetic switches offer light-dependent and reversible interactions, presenting an opportunity to innovate assay design.
Purpose of the Study:
- To introduce OptoAssay, a novel light-controlled diagnostic platform.
- To demonstrate a wash- and pump-free assay format for point-of-care applications.
- To showcase the versatility and enhanced sensitivity of the OptoAssay system.
Main Methods:
- Utilized optogenetic switches for light-dependent, reversible control of assay components.
- Implemented varying light wavelengths to direct bidirectional movement of assay elements.
- Applied the OptoAssay to calibrate a competitive model assay for detection.
Main Results:
- Achieved a superior limit of detection of 8 pg/mL, surpassing conventional ELISA tests.
- Demonstrated the dynamic bioassay format's efficacy across various substrates.
- Validated the light-controlled movement of assay components without pumps or valves.
Conclusions:
- OptoAssay circumvents limitations of traditional bioassays, enabling simpler, more efficient diagnostics.
- The technology holds potential for on-site analysis in resource-limited settings when integrated with smartphones.
- OptoAssay represents a significant advancement in developing dynamic, light-controlled diagnostic tools.
