Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nanotechnology to Break the Antimicrobial Resistance.

ACS infectious diseases·2026
Same author

Programmable Surface Catalyzed Heterogeneous Nucleation Enables "Double-Cable" Light-Harvesting Supramolecular Polymers.

Journal of the American Chemical Society·2026
Same author

Towards a context-aware framework for cellular senescence.

Biogerontology·2026
Same author

Engineering MoS<sub>2</sub>/CoSe<sub>2</sub> Hybrid Nanocomposites for Efficient Regeneration of Bacterially Infected Diabetic Wounds.

ACS applied bio materials·2026
Same author

Oxide interface-based polymorphic electronic devices for neuromorphic computing.

Nature communications·2026
Same author

Optically switchable CLEAR probes enable rapid, biocompatible and high-efficiency fluorophore exchange for ultra-plex, high-resolution immunofluorescence imaging.

Chemical science·2026

Related Experiment Video

Updated: May 11, 2026

Bacterial Detection & Identification Using Electrochemical Sensors
09:30

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

28.3K

Multistep Array-Based Sensing of Bioanalytes Using Modified MoS2, Fluorescence Proteins, and Cucurbituril.

Pradipta Behera1, Sourav Baidya1, Jagabandhu Sahoo1

  • 1Department of Organic Chemistry, Indian Institute of Science, Bangalore 560012, India.

ACS Applied Bio Materials
|September 25, 2024
PubMed
Summary

This study presents a novel one-pot sensor array for rapid bioanalyte discrimination using multiplexing strategies. The sensor effectively differentiates proteins and cell lines, demonstrating versatile detection capabilities.

Keywords:
2D MoS2biosensingcell lines and lysateshost−guest interactionsensor arrays

More Related Videos

FIBS-enabled Noninvasive Metabolic Profiling
09:16

FIBS-enabled Noninvasive Metabolic Profiling

Published on: February 3, 2014

9.8K
Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

7.8K

Related Experiment Videos

Last Updated: May 11, 2026

Bacterial Detection & Identification Using Electrochemical Sensors
09:30

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

28.3K
FIBS-enabled Noninvasive Metabolic Profiling
09:16

FIBS-enabled Noninvasive Metabolic Profiling

Published on: February 3, 2014

9.8K
Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

7.8K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Multiplexing in sensor arrays enables rapid discrimination of multiple analytes.
  • Existing methods often rely on multiple signal transducers or sequential agents.

Purpose of the Study:

  • To develop a one-pot sensor array combining multichannel and sequential strategies for bioanalyte discrimination.
  • To utilize molybdenum disulfide (MoS2) and fluorescent proteins for sensitive and selective detection.

Main Methods:

  • Constructed a sensor array using positively charged MoS2 as a receptor and fluorescent proteins as signal transducers.
  • Employed electrostatic interactions between MoS2 and Cucurbit [7] uril (CB7) to modulate fluorescence.
  • Utilized electrodynamic analysis and optical assays to investigate fluorescence modulation.

Main Results:

  • Successfully discriminated cationic and anionic proteins at 50 nM concentrations.
  • Achieved a detection limit of 1 nM for beta-galactosidase (β-gal) protein.
  • Demonstrated versatile detection by discriminating between normal and diseased cell lines and lysates.

Conclusions:

  • The developed sensor array offers a versatile platform for rapid bioanalyte discrimination.
  • Electrostatic interactions are crucial for modulating fluorescence outcomes in the array.
  • The sensor shows potential for applications in diagnostics and biological analysis.