Pesticide exposure patterns in honey bees during migratory pollination

Monitoring pesticide exposures in honey bees provides fundamental risk information that informs efforts to improve regulatory policy, pesticide use, and beekeeping management so pollinators are protected in realistic field conditions. We investigated pesticide exposures to bee colonies while colonies moved along commercial migratory routes in 2022 and 2023 to pollinate multiple pollinator-dependent, high-value U.S. specialty crops (e.g., almonds in California and apples and cherries in Washington). We found evident pesticide exposure patterns, including increasing exposures (both levels and number of pesticides) to fungicides during almond pollination, higher exposures to insecticides and persistent exposures to fungicides during springtime fruit pollination, and declining exposures in summer. Exposure risk assessment by risk quotient (RQ) model based on residues in bee bread indicates no concern of acute toxicity to adult honey bees during pollination, however, during colony inspections we found severe brood mortality in fields associated with high exposure to buprofezin, an insect growth regulator (IGR) thought to be safe for adult bees, which is permitted for use any time across the season. Our results suggest a need to improve compliance with insecticide label requirements during tree fruit pollination and a need for further research into the negative impacts of IGR on colony health especially on immature bees to inform potential policy changes.