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Determination of 4-n-butylresorcinol by fluorescence derivatization based on dopamine
Qing Liu1, Si Chen1, Yiming Nie1
1Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, China; Key Laboratory of Material Chemistry for Energy Conversion and Storage (HUST), Ministry of Education, China; School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 430074, Wuhan, China.
Abstract:
4-n-butylresorcinol (4nBR) is a frequently utilized as whitening ingredients in skincare cosmetics. Compared with other whitening ingredients, it can effectively inhibit tyrosinase with lower toxicity and superior inhibition efficacy. Under alkaline conditions, an induced oxidative coupling reaction can occur between 4nBR and dopamine (DA) to generate strong fluorescent substance azamonardine with an intense emission band centering at 476 nm when excited at 440 nm. This phenomenon can be used to establish a fluorescence analysis method for 4nBR. The results indicated that the linear range of the method was 1.0-24.0 nmol L-1, and the detection limit was as low as 0.25 nmol L-1. The method showed high sensitivity, good selectivity, mild experimental conditions and low cost. The proposed method was successfully used to detect 4nBR in cosmetics, and the results were consistent with those of HPLC. The spiking recoveries were between 98.2% and 108 %.
