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Updated: Jun 12, 2025

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach
Published on: March 12, 2017
Fateful Decisions of Where to Cut the Line: Pathology Associated with Aberrant 3' End Processing and Transcription
Pawel Grzechnik1, Hannah E Mischo2
1Division of Molecular and Cellular Function, School of Biological Sciences, University of Manchester, United Kingdom.
Abstract:
Aberrant gene expression lies at the heart of many pathologies. This review will point out how 3' end processing, the final mRNA-maturation step in the transcription cycle, is surprisingly prone to regulated as well as stochastic variations with a wide range of consequences. Whereas smaller variations contribute to the plasticity of gene expression, larger alternations to 3' end processing and coupled transcription termination can lead to pathological consequences. These can be caused by the local mutation of one gene or affect larger numbers of genes systematically, if aspects of the mechanisms of 3' end processing and transcription termination are altered.
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