Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proofreading01:43

Proofreading

53.9K
Overview
53.9K
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

9.9K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
9.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrating Bone-Brain Axis Modulation and Tea Consumption for Enhancing Neurovascular Resilience and Patient Rehabilitation Education.

Food science & nutrition·2026
Same author

Artificial Intelligence with Robotics for Metabolic Rehabilitation and Enhanced Patient Recovery in Critical Care.

Research (Washington, D.C.)·2026
Same author

Subtype-specific differences in susceptibility to monoclonal antibodies and vaccines among contemporary RSV-A and RSV-B isolates.

bioRxiv : the preprint server for biology·2026
Same author

Fully Modified SpyCas9 Guide RNAs Enable Robust Genome Editing In Cells and In Vivo.

bioRxiv : the preprint server for biology·2026
Same author

Transition from infectivity and immune escape to pure escape as an evolutionary strategy during the COVID-19 pandemic.

bioRxiv : the preprint server for biology·2026
Same author

High-Level Production of Ergothioneine in Yarrowia lipolytica via Systematic Metabolic Engineering Strategies.

Biotechnology and bioengineering·2026
Same journal

Unlocking the chemical potential of filamentous fungi using prime editing.

Nature biotechnology·2026
Same journal

A genome-scale CRISPRi perturbation atlas of human induced pluripotent stem cells.

Nature biotechnology·2026
Same journal

Prime editing for precise genome engineering and modulation of fungal metabolism.

Nature biotechnology·2026
Same journal

Retargeted serine integrases for one-step, precise integration of large DNA sequences in human cells.

Nature biotechnology·2026
Same journal

A retargeted recombinase for precise insertion of large DNA.

Nature biotechnology·2026
Same journal

Experiment-guided AlphaFold3 resolves measurement-consistent protein ensembles.

Nature biotechnology·2026
See all related articles

Related Experiment Video

Updated: Jun 12, 2025

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
15:22

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

17.0K

Increasing intracellular dNTP levels improves prime editing efficiency.

Pengpeng Liu1, Karthikeyan Ponnienselvan1, Thomas Nyalile2

  • 1Department of Molecular, Cell and Cancer Biology, University of Massachusetts Chan Medical School, Worcester, MA, USA.

Nature Biotechnology
|September 25, 2024
PubMed
Summary
This summary is machine-generated.

Prime editing efficiency is boosted by improving reverse transcriptase and increasing deoxynucleotide triphosphate (dNTP) levels. These combined strategies significantly enhance precise gene editing rates in cells.

More Related Videos

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

9.7K
Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
09:00

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies

Published on: May 22, 2012

403.6K

Related Experiment Videos

Last Updated: Jun 12, 2025

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
15:22

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

17.0K
Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

9.7K
Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
09:00

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies

Published on: May 22, 2012

403.6K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Intracellular deoxynucleotide triphosphate (dNTP) levels are critical for DNA replication and repair.
  • dNTP pools are tightly regulated in a cell cycle-dependent manner in primary cells.
  • Prime editing is a powerful gene editing technology with potential for therapeutic applications.

Purpose of the Study:

  • To investigate methods for enhancing prime editing efficiency.
  • To explore the impact of Moloney murine leukemia virus reverse transcriptase properties on prime editing.
  • To determine the effect of intracellular dNTP levels on prime editing outcomes.

Main Methods:

  • Utilized prime editing technology in primary cell types.
  • Introduced mutations to improve the enzymatic properties of Moloney murine leukemia virus reverse transcriptase.
  • Administered treatments to elevate intracellular dNTP levels.

Main Results:

  • Mutations enhancing reverse transcriptase enzymatic properties increased prime editing efficiency.
  • Treatments increasing intracellular dNTP levels also boosted prime editing efficiency.
  • Combining these modifications led to substantial increases in precise editing rates.

Conclusions:

  • Optimizing reverse transcriptase and increasing dNTP levels are effective strategies to enhance prime editing.
  • These combined approaches offer a promising avenue for improving the precision and efficacy of gene editing.
  • Further research can leverage these findings for advanced genetic engineering applications.