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Phenotypic changes and gene expression profiles of Vibrio parahaemolyticus in response to low concentrations of
Xi Luo1, Miaomiao Zhang1, Yiquan Zhang2
1Department of Clinical Laboratory, Nantong Third People's Hospital, Affiliated Nantong Hospital 3 of Nantong University, Nantong, Jiangsu, China.
Abstract:
Vibrio parahaemolyticus is a leading cause of seafood-associated gastroenteritis and possesses intrinsic resistance to ampicillin. While ampicillin can trigger transcriptional responses of global genes, the behavioral and molecular changes that occur in V. parahaemolyticus when exposed to ampicillin are not fully understood. In this work, we investigated the effects of low concentrations of ampicillin on the physiology and gene expression of V. parahaemolyticus by combining phenotypic assays and RNA sequencing (RNA-seq) analysis. Our results showed that the growth of V. parahaemolyticus were notably delayed, and both motility and c-di-GMP production were significantly inhibited in the response to low concentrations of ampicillin stress. In contrast, biofilm formation by V. parahaemolyticus was enhanced by exposure to low concentrations of ampicillin. However, low concentrations of ampicillin had no effect on the cytotoxicity or adherence activity of V. parahaemolyticus. The RNA-seq data revealed that a low concentration of ampicillin significantly affected the expression levels of 676 genes, including those involved in antibiotic resistance, virulence, biofilm formation, and regulation. This work contributes to our understanding of how V. parahaemolyticus alters its behavior and gene expression in response to ampicillin exposure.
Insights
Low ampicillin concentrations inhibit Vibrio parahaemolyticus growth and motility but enhance biofilm formation. This study reveals significant gene expression changes in response to ampicillin stress.
Area of Science:
- Microbiology
- Molecular Biology
- Genomics
Background:
- Vibrio parahaemolyticus causes seafood-associated gastroenteritis and is intrinsically resistant to ampicillin.
- Ampicillin exposure triggers global gene transcriptional responses, but detailed physiological and molecular changes remain unclear.
Purpose of the Study:
- To investigate the physiological and gene expression effects of low ampicillin concentrations on Vibrio parahaemolyticus.
- To elucidate the behavioral and molecular alterations in V. parahaemolyticus upon ampicillin exposure.
Main Methods:
- Phenotypic assays were employed to assess bacterial physiology.
- RNA sequencing (RNA-seq) was utilized for comprehensive gene expression analysis.
Main Results:
- Low ampicillin concentrations significantly inhibited V. parahaemolyticus growth, motility, and cyclic-di-GMP (c-di-GMP) production.
- Biofilm formation was enhanced, while cytotoxicity and adherence remained unaffected by low ampicillin concentrations.
- RNA-seq identified 676 differentially expressed genes, including those related to antibiotic resistance, virulence, and biofilm formation.
Conclusions:
- Low ampicillin concentrations induce significant physiological and gene expression changes in V. parahaemolyticus.
- The findings provide insights into V. parahaemolyticus adaptation mechanisms and gene regulation under antibiotic stress.
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