Characterization of ATP-binding cassette transporters associated with emamectin benzoate tolerance: from the model insect Drosophila melanogaster to the agricultural pest Spodoptera frugiperda
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View abstract on PubMed
Summary
This summary is machine-generated.This study identifies nine ATP-binding cassette (ABC) transporter genes involved in emamectin benzoate (EB) tolerance in insects. The findings validate a
Area Of Science
- Insect toxicology
- Molecular biology
- Genetics
Background
- Insecticide resistance is a growing problem managed by detoxification genes.
- ATP-binding cassette (ABC) transporters are crucial in detoxifying synthetic insecticides.
- Identifying all relevant genes is key for effective insecticide resistance management.
Purpose Of The Study
- To test a 'model to pest' strategy for identifying ABC transporters related to emamectin benzoate (EB) tolerance.
- To identify EB-tolerance-related ABC transporter genes in the agricultural pest Spodoptera frugiperda.
- To confirm the role of ABC transporters in EB toxicity using Drosophila melanogaster as a model.
Main Methods
- Functional screening using RNA interference (RNAi) in Drosophila melanogaster.
- Phylogenetic analysis and reciprocal TBLASTN searches to identify homologous genes in Spodoptera frugiperda.
- Real-time quantitative PCR (qPCR) to analyze gene expression.
- Cytotoxicity assays using ABC-overexpressing Sf9 cell lines.
Main Results
- Four ABC transporter genes in D. melanogaster were linked to EB tolerance.
- Five homologous ABC transporter genes were identified in S. frugiperda.
- All five S. frugiperda ABC transporter genes were inducible by EB and expressed across developmental stages and tissues.
- Overexpression of these five genes conferred EB tolerance in Sf9 cells.
Conclusions
- Nine ABC transporter genes (four from D. melanogaster, five from S. frugiperda) are associated with EB tolerance.
- The 'model to pest' strategy is effective for identifying toxicity-related genes.
- This approach aids in understanding and managing insecticide resistance.
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