Related Experiment Video

Updated: Jun 12, 2025

The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

111.5K

ATF6 Promotes Colorectal Cancer Growth and Stemness by Regulating the Wnt Pathway

Jeffrey J Rodvold1, Matthew Grimmer2, Karen Ruiz3

  • 1Department of Research Oncology, Genentech, Inc., South San Francisco, California.

Cancer Research Communications
|September 26, 2024
PubMed
Abstract

Insights

Activating transcription factor 6 (ATF6) reduces colorectal cancer cell viability by disrupting Wnt signaling. This identifies ATF6 as a potential therapeutic target for colorectal cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Colorectal cancer (CRC) is a major global health concern.
  • Dysregulated Wnt signaling is a hallmark of CRC development and progression.
  • Novel therapeutic targets are needed to improve CRC treatment outcomes.

Purpose of the Study:

  • To investigate the role of Activating Transcription Factor 6 (ATF6) in colorectal cancer.
  • To determine if ATF6 can modulate Wnt signaling in CRC cells.
  • To evaluate ATF6 as a potential therapeutic target for colorectal cancer.

Main Methods:

  • Utilized cell viability assays in colorectal cancer cell lines.
  • Employed organoid models to assess the in vivo relevance of ATF6.
  • Investigated the impact of ATF6 on Wnt signaling pathway components.

Main Results:

  • ATF6 intervention significantly reduced colorectal cancer cell viability.
  • ATF6 inhibition decreased the viability of colorectal cancer organoids.
  • ATF6 activity was found to interrupt dysregulated Wnt signaling in CRC.

Conclusions:

  • ATF6 plays a crucial role in promoting colorectal cancer cell survival and growth.
  • Targeting ATF6 presents a novel therapeutic strategy for colorectal cancer.
  • Interruption of Wnt signaling by ATF6 highlights its potential as a therapeutic target.

More Related Videos

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres06:52

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

6.5K
In vitro Organoid Culture of Primary Mouse Colon Tumors07:33

In vitro Organoid Culture of Primary Mouse Colon Tumors

Published on: May 17, 2013

35.0K

Related Concept Videos

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.7K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.2K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
4.9K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K