Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

875
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
875
Leaky Scanning02:28

Leaky Scanning

5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

13.5K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.5K
Experimental RNAi02:15

Experimental RNAi

6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

5.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
5.6K
What is Gene Expression?01:36

What is Gene Expression?

8.5K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
8.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The role of TRPV1 in corneal wound healing under a thyroxine-induced TAO-like condition.

Experimental eye research·2026
Same author

Covalent Amorphous Alumina-Hydrogenated Graphene Materials With Integrated Proton Radiation Shielding and Energy Storage Capability for Space Electronics.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Schneiderian membrane perforation upon the advancement of mechanical drills passing through sinus walls: An <i>ex vivo</i> animal study.

Journal of dental sciences·2026
Same author

Biofunctionalized 3D-printed gelatin-alginate scaffolds with arginine-glycine-aspartic acid (RGD) peptides for enhanced <i>in vitro</i> osteogenesis.

Journal of dental sciences·2026
Same author

One-dimensional dielectric grating structure for plasmonic coupling and routing.

Nanophotonics (Berlin, Germany)·2025
Same author

Monitoring Adherence and Renal Safety of Nucleos(t)ide Analogs for Patients With Chronic Hepatitis B.

Clinical and translational gastroenterology·2025

Related Experiment Video

Updated: Jun 12, 2025

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

8.8K

Exogenous Janus Kinase 617 Codon Influences Small Noncoding RNAs and Gene Expression in Ba/F3 Cells.

Yi-Yang Chen1, Ying-Hsuan Wang1,2, Chih-Cheng Chen1,2

  • 1Division of Hematology and Oncology, Chang Gung Memorial Hospital, Chiayi, Taiwan.

Journal of Physiological Investigation
|September 26, 2024
PubMed
Summary

The JAK2V617F mutation in myeloproliferative neoplasms (MPNs) can rearrange the epigenome, affecting microRNA and PIWI-interacting RNA expression. This study clarifies how this mutation drives MPN transformation through the JAK-STAT pathway.

More Related Videos

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

10.8K
Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells
10:21

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Published on: January 5, 2018

13.1K

Related Experiment Videos

Last Updated: Jun 12, 2025

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

8.8K
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

10.8K
Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells
10:21

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Published on: January 5, 2018

13.1K

Area of Science:

  • Hematology
  • Molecular Biology
  • Genetics

Background:

  • Myeloproliferative neoplasms (MPNs) are blood cancers often associated with the JAK2V617F mutation.
  • The precise molecular mechanisms by which JAK2V617F drives MPN transformation remain unclear.
  • Dysregulation of noncoding RNAs (ncRNAs) like microRNA (miRNA) and PIWI-interacting RNA (piRNA) is observed in MPNs, but direct causality by JAK2V617F is unproven.

Purpose of the Study:

  • To investigate the direct molecular effects of the JAK2V617F mutation on gene and ncRNA expression.
  • To determine if JAK2V617F alone can induce epigenetic alterations and impact the JAK-STAT signaling pathway.
  • To elucidate the mechanisms underlying MPN transformation driven by JAK2V617F.

Main Methods:

  • Exogenous expression of wild-type JAK2 and JAK2V617F in mouse Ba/F3 cells.
  • Next-generation sequencing of small and total RNAs to analyze differential expression.
  • Pathway analysis to identify affected signaling cascades and epigenetic modifications.

Main Results:

  • Significant differences in miRNA and gene expression were observed between cells expressing wild-type JAK2 and JAK2V617F.
  • Differentially expressed variations included enriched transposable elements and piRNAs, suggesting epigenetic rearrangement.
  • Pathway analysis confirmed the involvement of the JAK-STAT signaling pathway in the transformation process.

Conclusions:

  • JAK2V617F directly induces alterations in miRNA and piRNA expression, leading to epigenetic changes.
  • The JAK-STAT pathway is a key mediator of JAK2V617F-induced transformation.
  • These findings provide a clearer understanding of the molecular mechanisms driving MPN development.