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Updated: Jun 17, 2026

Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer
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Optimizing the Structure of a Pt Metal-Chelating Polymer to Reduce Nonspecific Binding for Mass Cytometry

Edmond C N Wong1, Yefeng Zhang1, Tianjia Yang1

  • 1Department of Chemistry, University of Toronto, Toronto, Ontario M5S 3H6, Canada.

Biomacromolecules
|September 26, 2024
PubMed

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Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is formed in...
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