Advanced roll porous scaffold 3D bioprinting technology

Area of Science:

• Biotechnology
• Regenerative Medicine
• Tissue Engineering

Background:

• Current 3D bioprinting methods face limitations in speed, volume, and cell density.
• Precise cell placement and preventing leakage remain significant challenges in biomanufacturing.
• The need for advanced biomodels for drug testing and personalized medicine is increasing.

Purpose of the Study:

• To introduce and evaluate the roll porous scaffold (RPS) 3D bioproduction technology.
• To demonstrate improvements in cell print density, organoid formation purity, and biomanufacturing speed and volume.
• To highlight the potential of RPS technology for drug screening and personalized medicine applications.

Main Methods:

• Utilized roll porous scaffold (RPS) technology with 360 and 1200 dpi inkjet printheads.
• Engineered a spongy, bioresorbable ribbon for precise cell placement and enhanced bioprinting.
• Developed 3D biomodels for 'organ on a chip' applications and personalized medicine.

Main Results:

• Achieved a ~20% increase in print density for 10-15 µm cells, reaching up to 1.5 × 10^8 cells/mL.
• Improved organoid formation purity by >17% using the RPS technology.
• Demonstrated biomanufacturing performance exceeding 1.8 L/h for 15 µm layer thickness, surpassing existing 3D bioprinting methods.

Conclusions:

• RPS 3D bioproduction technology offers superior speed, volume, and print density compared to dominant methods, without requiring expensive equipment.
• The technology enables efficient 'organ on a chip' models for non-animal substance testing and personalized medicine.
• RPS technology facilitates the creation of personalized 3D organoids for targeted therapy, drug selection, and overcoming organ shortages through DNA-based printed endocrine glands.