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Published on: June 9, 2014
Colorimetric screening model for identification of menaquinone-7 (MK-7) producing strains
Qiu-Hua Zhang1, Zheng Wang1, Yao-Qiang Wang1
1Beijing Key Laboratory of Bioprocess, and Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029 People's Republic of China.
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In this study, a novel colorimetric screening method for identifying menaquinone-7 (MK-7) producing strains was established using potassium permanganate. To our knowledge, this method represents the first direct screening methodology for the identification of MK-7 producing strains. Utilizing this screening method, a new MK-7 producing strain, Bacillus subtilis GSA-184, was identified from the soil of the Tibetan Plateau. Under the optimized fermentation medium (50 g/L glycerol, 30 g/L yeast extract powder, 100 g/L soybean peptone, 1 g/L KH2PO4, and 1 g/L MnSO4), the production of MK-7 was increased to 25.7 mg/L. Additionally, the maximum production of MK-7 reached 36.46 mg/L after 48 h in a 5-L fermenter.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s13205-024-04097-1.
