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High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

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Spectrochip-based Calibration Curve Modeling (CCM) for Rapid and Accurate Multiple Analytes Quantification in

Cheng-Hao Ko^1,2, Ashenafi Belihu Tadesse¹, Abel Chernet Kabiso¹

¹Graduate Institute of Automation and Control, National Taiwan University of Science and Technology, Taipei, Taiwan.

|September 27, 2024

Summary

This study introduces a spectrochip device for rapid and accurate quantification of twelve urine test strip parameters. This micro-electromechanical system (MEMS) technology enables convenient point-of-care urinalysis, reducing reliance on large testing machines.

Keywords:

Calibration Curve Modeling (CCM)Characteristic Wavelength (Micro-electromechanical System (MEMS)Point-of-Care (POC) Testing Spectrochip Urinalysis

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Area of Science:

Biomedical Engineering
Analytical Chemistry
Clinical Diagnostics

Background:

Traditional urine test strips offer semi-quantitative results, susceptible to judgmental errors.
Accurate and rapid multi-parameter urinalysis is crucial for diagnosing renal disorders.
Existing diagnostic methods often require large, time-consuming laboratory equipment.

Purpose of the Study:

To develop and validate a micro-electromechanical system (MEMS)-based spectrophotometer (spectrochip) for accurate quantification of twelve urine parameters.
To demonstrate the spectrochip's capability for routine, multi-parameter urine analysis.
To assess the potential of the spectrochip for point-of-care (POC) testing.

Main Methods:

Absorption spectra of eight concentration levels for twelve urine parameters were measured independently.
Calibration curves were established using average spectral intensities at characteristic wavelengths.
Regression analysis was performed using GraphPad Prism to determine the coefficient of determination (R²).

Main Results:

The spectrochip accurately quantified twelve urine parameters, including leukocytes, protein, glucose, and creatinine.
Modeled calibration curves demonstrated a coefficient of determination (R²) greater than 0.95, indicating high accuracy.
The spectrochip enables direct comparison with calibration curves, even for unknown sample concentrations.

Conclusions:

The spectrochip provides a rapid, accurate, and reliable method for multi-parameter urinalysis.
This MEMS-based platform facilitates convenient point-of-care (POC) testing, reducing costs and time.
The spectrochip technology offers a significant advancement over traditional semi-quantitative urine test strips.