Analysis of Random Lasing in Human Blood

Area of Science:

• Optics
• Biomedical Engineering
• Hematology

Background:

• Random lasing (RL) combines light amplification and scattering for feedback.
• Investigating RL in biological samples like human blood is a developing field.
• Blood diseases like Chronic Lymphocytic Leukemia (CLL) present diagnostic challenges.

Purpose of the Study:

• To explore random lasing generation in human blood components.
• To assess the potential of RL signal analysis for diagnosing blood diseases, specifically CLL.
• To develop a classification method for blood samples using RL spectra.

Main Methods:

• Prepared mixtures of rhodamine B dye with blood components (platelets, lymphocytes, erythrocytes, whole blood).
• Induced and measured random lasing spectra from these mixtures.
• Applied statistical analyses (Principal Component Analysis, Linear Discriminant Analysis) to RL spectra for sample classification.
• Tested samples from healthy individuals and CLL patients.

Main Results:

• Intense coherent RL was achieved in blood components (except erythrocytes) at low pump thresholds.
• RL-based discrimination between healthy and CLL samples was successful.
• Classification accuracy reached 86.7% for lymphocyte samples.
• RL spectra showed distinct patterns for different blood components and disease states.

Conclusions:

• Random lasing is feasible in various human blood components.
• RL analysis demonstrates significant potential as a non-invasive biosensing tool for blood diagnostics.
• This technique shows promise for early detection and monitoring of blood disorders like CLL.