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Paper-Based Microfluidic Device for Extracellular Lactate Detection.

Yan Nan1, Peng Zuo1, Bangce Ye1

  • 1Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, China.

Biosensors
|September 27, 2024
PubMed
Summary
This summary is machine-generated.

This study presents a rapid, paper-based biosensor for detecting tumor-secreted lactate, aiding early cancer diagnosis and drug screening. The low-cost platform offers sensitive lactate analysis for cell lines, valuable in resource-limited settings.

Keywords:
colorimetric analysisdrug screeninglactate detectionpaper-based microfluidictumor cell

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Area of Science:

  • Biomedical Engineering
  • Cancer Research
  • Analytical Chemistry

Background:

  • Tumor-secreted lactate significantly impacts cancer progression, metastasis, and patient outcomes.
  • Accurate measurement of tumor-derived lactate is crucial for early cancer detection.
  • Existing methods for lactate analysis can be complex and costly.

Purpose of the Study:

  • To develop a rapid, sensitive, and cost-effective paper-based microfluidic chip for detecting lactate secreted by living cells.
  • To enable precise analysis of lactate levels for early cancer diagnosis and antitumor drug screening.
  • To create a platform applicable in resource-constrained environments.

Main Methods:

  • A paper-based microfluidic chip was designed for external lactate detection from cell cultures.
  • Enzymatic chemical conversion was employed to generate fluorescent signals proportional to lactate concentration.
  • Qualitative assessment used a handheld UV lamp, and quantitative analysis utilized ImageJ software for grayscale intensity measurements.

Main Results:

  • The lactate biosensor achieved assay completion in under 30 minutes under optimized conditions.
  • The platform demonstrated the ability to differentiate lactate secretion levels across various cancer cell lines.
  • The system provided both qualitative and quantitative lactate measurements with high sensitivity.

Conclusions:

  • The developed paper-based microfluidic platform offers a rapid, sensitive, and affordable method for analyzing cell-secreted lactate.
  • This technology is valuable for early cancer diagnosis, understanding tumor-lactate interactions, and high-throughput screening of anti-cancer drugs.
  • Its simplicity and low cost make it suitable for use in resource-limited settings, advancing cancer research and diagnostics.