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Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Updated: Apr 14, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

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Published on: November 20, 2013

Electrochemical Impedance Spectroscopy-Based Microfluidic Biosensor Using Cell-Imprinted Polymers for Bacteria

Shiva Akhtarian¹, Satinder Kaur Brar², Pouya Rezai¹

¹Department of Mechanical Engineering, York University, Toronto, ON M3J 1P3, Canada.

|September 27, 2024

Summary

This summary is machine-generated.

This study introduces a new biosensor using cell-imprinted polymers on microwires for detecting E. coli bacteria in water. The portable sensor offers sensitive, rapid, and cost-effective water quality monitoring at the point-of-need.

Keywords:

bacteria detection biosensor cell-imprinted polymer (CIP)electrochemical sensor microfluidic

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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Area of Science:

Environmental Science
Analytical Chemistry
Materials Science

Background:

Point-of-need (PoN) biosensors are crucial for real-time water quality monitoring.
Cell-imprinted polymers (CIPs) offer specific bacterial cell capture but require enhanced sensitivity.
Integrating CIPs into microfluidic devices presents challenges in improving detection limits.

Purpose of the Study:

To develop and validate a novel biosensor for sensitive impedimetric detection of E. coli.
To integrate cell-imprinted polymer-functionalized stainless steel microwires (CIP-MWs) into a microfluidic device.
To assess the performance of the developed biosensor for waterborne pathogen detection.

Main Methods:

Fabrication of CIP-functionalized stainless steel microwires (CIP-MWs) and non-imprinted polymer microwires (NIP-MWs).
Integration of CIP-MWs and NIP-MWs into a dual-channel microfluidic device for electrochemical impedance spectroscopy (EIS).
Impedimetric detection of E. coli by measuring changes in charge transfer resistance before and after bacterial incubation.

Main Results:

The CIP-MW biosensor showed increased charge transfer resistance upon E. coli incubation.
A limit of detection (LOD) of 2 × 10^2 CFU/mL and a limit of quantification (LOQ) of 1.4 × 10^4 CFU/mL were determined.
The sensor exhibited a dynamic detection range from 10^2 to 10^7 CFU/mL for E. coli.

Conclusions:

The developed CIP-MW based microfluidic biosensor provides a sensitive and rapid method for E. coli detection.
This cost-effective and portable sensor is suitable for on-site water quality monitoring.
The study demonstrates the potential of CIPs integrated with microfluidic impedance sensors for detecting waterborne pathogens.