Related Experiment Video

Updated: Jul 13, 2026

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
09:13

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

17.4K

Let-7f-5p Modulates Lipid Metabolism by Targeting Sterol Regulatory Element-Binding Protein 2 in Response to PRRSV

Dongfeng Jiang1,2,3, Liyu Yang2,3, Xiangge Meng1

  • 1Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.

Veterinary Sciences
|September 27, 2024
PubMed

Insights

Porcine reproductive and respiratory syndrome virus (PRRSV) induces the microRNA let-7f-5p, which impacts lipid metabolism by targeting SREBP2 to regulate disease progression. This finding offers a potential new antiviral target for PRRS.

Area of Science:

  • Veterinary Virology
  • Molecular Biology
  • Lipid Metabolism

Background:

  • Porcine reproductive and respiratory syndrome (PRRS) significantly impacts the swine industry.
  • MicroRNAs (miRNAs) are recognized as key regulators in the pathogenesis of PRRS virus (PRRSV).

Purpose of the Study:

  • To investigate the role of let-7f-5p in PRRSV pathogenesis.
  • To elucidate the molecular mechanisms by which let-7f-5p influences PRRSV infection, focusing on lipid metabolism.

Main Methods:

  • Transcriptome analysis of PRRSV-infected cells with altered let-7f-5p levels.
  • Dual-luciferase reporter assay to confirm direct targeting of SREBP2 by let-7f-5p.
  • Western blotting to assess SREBP2 protein levels.

Main Results:

  • PRRSV infection induces let-7f-5p expression.
  • Transcriptome analysis revealed significant alterations in lipid metabolism pathways.
  • let-7f-5p directly targets and downregulates the lipogenesis master regulator SREBP2.
  • let-7f-5p modulates lipogenesis in PRRSV-infected cells.

Conclusions:

  • let-7f-5p plays a critical role in PRRSV pathogenesis by modulating lipid metabolism through SREBP2.
  • This miRNA-mediated pathway represents a novel insight into PRRSV infection.
  • let-7f-5p targeting of SREBP2 presents a potential therapeutic target for PRRSV.
Keywords:
PRRSVRNA-seqSREBP2let-7f-5plipogenesis

More Related Videos

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells08:58

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

Published on: May 1, 2019

14.8K
Author Spotlight: Exploring Cloning Techniques for Full-Length DNA Fragments04:18

Author Spotlight: Exploring Cloning Techniques for Full-Length DNA Fragments

Published on: May 17, 2024

551

Related Concept Videos

Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
Lipid Catabolism01:25

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
Regulation of Bacterial Virulence01:28

Regulation of Bacterial Virulence

Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...
Respiratory Syncytial Virus Disease01:29

Respiratory Syncytial Virus Disease

Human respiratory syncytial virus (RSV) is a widespread pathogen that primarily targets infants and young children but also poses a serious health risk to elderly and immunocompromised individuals. Belonging to the Pneumoviridae family, RSV is a negative-sense, single-stranded RNA virus within the Pneumovirus genus. Its global health burden is significant, with millions of cases annually resulting in hospitalizations and mortality, particularly in resource-limited settings. Although most...