Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Transgenic Organisms

Transgenic Organisms

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Decoding and vocabulary improvements mediate sustained gains in reading comprehension: Evidence from a randomised controlled trial of a multicomponent reading intervention.

Journal of child psychology and psychiatry, and allied disciplines·2026

Same author

How Orthographic Transparency Shapes Word Learning and Retention in Monolingual and Bilingual Adults.

Quarterly journal of experimental psychology (2006)·2026

Same author

Procedural eptifibatide administration as an alternative to preprocedural dual antiplatelet therapy for patients undergoing flow diversion procedures: case series and review of the literature.

Journal of neurointerventional surgery·2025

Same author

Effects of mental health on transition readiness in primary care.

Health care transitions·2025

Same author

Distribution of <i>Rachiplusia nu</i> and <i>Chrysodeixis includens</i> in <i>Bt</i> and Conventional Soybean Fields in Brazil.

Insects·2025

Same author

A Novel Polymerase Chain Reaction (PCR)-Based Method for the Rapid Identification of <i>Chrysodeixis includens</i> and <i>Rachiplusia nu</i>.

Insects·2025

Same journal

Chemical, Biological, and Ecological Evidence for Aerobic Deoxynivalenol Detoxification in Agronomic Soil-Derived Bacterial Communities.

Toxins·2026

Same journal

Botulinum Toxin Treatment for Uncommon Phenotypes of Laryngeal Adductor Breathing Dystonia.

Toxins·2026

Same journal

Enhancing Neuronal Networks with <i>Rhinella schneideri</i> Skin Secretion Molecules: Implications for Neurodegenerative Disorders.

Toxins·2026

Same journal

Dangerous Measures: A Case Report and Review of Motoro Ray Envenomation.

Toxins·2026

Same journal

The Impact of OnabotulinumtoxinA on Oral Pain Medication Prescription Fills and Low-Value Care in Patients with Cervical Dystonia in the United States: A Retrospective Claims Analysis.

Toxins·2026

Same journal

Broad-Spectrum Antiviral and Antibacterial Activity of the Scorpion Venom Peptide HP1090.

Toxins·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 11, 2025

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata

Published on: October 1, 2016

Utility of Cry1Ja for Transgenic Insect Control.

John P Mathis¹, Catherine Clark¹, Amit Sethi¹

¹Corteva Agriscience, Indianapolis, IN 46268, USA.

|September 27, 2024

Summary

This summary is machine-generated.

New insecticidal proteins like Cry1Ja offer novel modes of action to combat pest resistance. This research evaluates Cry1Ja

Keywords:

Bacillus thuringiensis Chrysodeixis includens Cry1J protein Helicoverpa zea Spodoptera frugiperda competitive binding assay in planta assay insecticidal protein bioassay

More Related Videos

RNAi Trigger Delivery into Anopheles gambiae Pupae

RNAi Trigger Delivery into Anopheles gambiae Pupae

Published on: March 8, 2016

Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

Published on: April 27, 2018

Related Experiment Videos

Last Updated: Jun 11, 2025

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata

Published on: October 1, 2016

RNAi Trigger Delivery into Anopheles gambiae Pupae

RNAi Trigger Delivery into Anopheles gambiae Pupae

Published on: March 8, 2016

Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

Published on: April 27, 2018

Area of Science:

Agricultural Entomology
Molecular Biology
Biochemistry

Background:

Bacillus thuringiensis (Bt) proteins are crucial for insect pest management and crop yield.
Insect resistance to current Bt traits necessitates novel insecticidal proteins with different modes of action.
Cry1Ja demonstrates insecticidal activity and in planta protection against lepidopteran pests.

Purpose of the Study:

To evaluate the mode of action (MoA) of the Cry1Ja protein.
To compare Cry1Ja's binding sites with existing Bt proteins in key lepidopteran pests.
To assess Cry1Ja's efficacy against insect populations resistant to other Bt toxins.

Main Methods:

Competitive binding assays using brush border membrane vesicles (BBMVs) from Helicoverpa zea, Spodoptera frugiperda, and Chrysodeixis includens.
Evaluation of Cry1Ja's bioactivity against Cry1Fa-resistant fall armyworm (FAW).
Assessment of in planta efficacy of Cry1Ja against target pests.

Main Results:

Cry1Ja exhibits distinct binding site interactions compared to other commercial Bt proteins in tested insect species.
Cry1Ja demonstrates significant bioactivity against fall armyworm populations resistant to Cry1Fa.
In planta expression of Cry1Ja provides robust protection against insect feeding.

Conclusions:

Cry1Ja possesses a unique mode of action, making it a valuable candidate for new insect trait development.
Cry1Ja can overcome existing resistance mechanisms in lepidopteran pests.
The findings support the development of novel Bt-based insect control strategies.