Related Experiment Video
Updated: Jun 12, 2025
In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model
Published on: October 3, 2011
Protocol to study oxygen dynamics in the in vivo mouse brain using bioluminescence microscopy
Antonios Asiminas1, Ryszard S Gomolka1, Stefanie Gregoriades1
1Division of Glial Disease and Therapeutics, Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
We developed a novel bioluminescence imaging protocol to measure oxygen levels in mouse brains. This method accurately maps hypoxic pockets with high spatiotemporal resolution.
Area of Science:
- Neuroscience
- Biochemistry
- Medical Imaging
Background:
- Bioluminescence imaging (BLI) utilizes enzyme-substrate reactions for light emission.
- Understanding molecular oxygen dynamics in the brain is crucial for neurological research.
- Current methods for measuring brain oxygenation have limitations.
Purpose of the Study:
- To present a novel protocol for studying in vivo molecular oxygen dynamics in the mouse brain.
- To utilize an oxygen-dependent bioluminescence reaction for enhanced imaging.
- To provide a method with superior spatiotemporal properties compared to existing techniques.
Main Methods:
- Developed a protocol involving acute craniotomy in mice.
- Employed viral transfection for enzyme delivery.
- Administered luciferase substrate for imaging.
- Utilized bioluminescence imaging to analyze oxygen-dependent light emission.
- Focused on identifying and analyzing hypoxic pockets.
Main Results:
- Successfully established a protocol for in vivo oxygen dynamics measurement in the mouse brain.
- Demonstrated the capability to image hypoxic pockets with high resolution.
- The developed BLI protocol exhibits superior spatiotemporal properties.
Conclusions:
- The presented protocol offers a powerful new tool for studying brain oxygenation.
- This method provides a significant advancement over traditional approaches like electrodes and phosphorescence.
- Enables detailed investigation of molecular oxygen dynamics in neurological contexts.
More Related Videos
18:40Combined In vivo Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice
Published on: October 16, 2014
08:58Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
Published on: May 4, 2011