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Updated: Jun 11, 2025

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
Published on: May 13, 2017
Fluorescence-Detected Two-Dimensional Electronic Spectroscopy of a Single Molecule
Sanchayeeta Jana1, Simon Durst1, Markus Lippitz1
1Experimental Physics III, University of Bayreuth, 95447 Bayreuth, Germany.
Abstract:
Single-molecule fluorescence spectroscopy is a powerful method that avoids ensemble averaging, but its temporal resolution is limited by the fluorescence lifetime to nanoseconds at most. At the ensemble level, two-dimensional spectroscopy provides insight into ultrafast femtosecond processes, such as energy transfer and line broadening, even beyond the Fourier limit, by correlating pump and probe spectra. Here, we combine these two techniques and demonstrate coherent 2D spectroscopy of individual dibenzoterrylene (DBT) molecules at room temperature. We excite the molecule in a confocal microscope with a phase-modulated train of femtosecond pulses and detect the emitted fluorescence with single-photon counting detectors. Using a phase-sensitive detection scheme, we were able to measure the nonlinear 2D spectra of most of the DBT molecules that we studied. Our method is applicable to a wide range of single emitters and opens new avenues for understanding energy transfer in single quantum objects on ultrafast time scales.
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