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Updated: Jun 11, 2025

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
Published on: July 29, 2015
Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation
Hongmei Xin1, Yixiao Li2, Kaihui Zhang1
1Pediatric Research Institute, Children's Hospital Affiliated to Shandong University, Jinan 250022, China.
Abstract:
A human induced pluripotent stem cell (iPSC) line was generated from patient with type 1 neurofibromatosis (NF-1), carrying heterozygous mutation in NF1 gene. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating delivery of KFL4, OCT4, SOX2, BCL-XL and c-MYC. The iPSC line expresses pluripotency markers, displays a normal karyotype, and is able to differentiate into three germ layers in vitro. This iPSC line represents a valuable cell model for NF1 in humans.

