Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A clinical evaluation of amlexanox oral adhesive pellicles in the treatment of recurrent aphthous stomatitis and comparison with amlexanox oral tablets: a randomized, placebo controlled, blinded, multicenter clinical trial.

Trials·2009
Same author

Long-term assessment of bladder and bowel dysfunction after radical hysterectomy.

Gynecologic oncology·2009
Same author

Oxidative stress contributes to silica nanoparticle-induced cytotoxicity in human embryonic kidney cells.

Toxicology in vitro : an international journal published in association with BIBRA·2009
Same author

A rapid and simple method for identifying Mycobacterium tuberculosis W-Beijing strains based on detection of a unique mutation in Rv0927c by PCR-SSCP.

Microbes and infection·2009
Same author

CO oxidation over AuPd(100) from ultrahigh vacuum to near-atmospheric pressures: the critical role of contiguous Pd atoms.

Journal of the American Chemical Society·2009
Same author

Daunorubicin-loaded magnetic nanoparticles of Fe(3)O(4) greatly enhance the responses of multidrug-resistant K562 leukemic cells in a nude mouse xenograft model to chemotherapy.

Zhongguo shi yan xue ye xue za zhi·2009

Related Experiment Video

Updated: Jun 11, 2025

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

9.4K

High-throughput single cell analysis using multi-focus Raman spectroscopy under randomly interleaved scattering

Hang Yuan1, Guiwen Wang2, Pengfei Zhang1

  • 1School of Precision Instrument and Opto-electronics Engineering, Tianjin University, Tianjin 300072, China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|September 27, 2024
PubMed
Summary

This study introduces a novel multi-focus Raman spectroscopy technique to significantly speed up single-cell analysis. The method enhances throughput by up to 15 times, enabling efficient, label-free monitoring of cellular dynamics.

Keywords:
Compressive sensingMulti-focus excitationRaman microspectroscopySingle-cell dynamics

More Related Videos

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

3.9K
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.0K

Related Experiment Videos

Last Updated: Jun 11, 2025

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

9.4K
Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

3.9K
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.0K

Area of Science:

  • Biophotonics
  • Spectroscopy
  • Cell Biology

Background:

  • Raman microspectroscopy offers label-free monitoring of single-cell dynamics.
  • Traditional methods are inefficient due to single-point acquisition, analyzing only one cell at a time.

Purpose of the Study:

  • To develop a high-throughput method for label-free single-cell analysis.
  • To overcome the inefficiency of traditional Raman microspectroscopy for dynamic cellular studies.

Main Methods:

  • Combines multi-focus Raman excitation with random interleaving of scattering projections.
  • Utilizes a time-sharing multi-focus array for simultaneous multi-cell excitation.
  • Employs compressive sensing to reconstruct individual cell spectra from mixed signals.

Main Results:

  • Achieved a throughput increase of up to 15 times for single-cell analysis.
  • Demonstrated high fidelity in reconstructed spectra, accurately capturing cellular changes.
  • Validated through numerical modeling and experimental investigation of spore germination.

Conclusions:

  • The developed technology enables high-throughput, label-free monitoring of living single cells.
  • Paves the way for advanced applications in cell biology research.
  • Offers a significant advancement over conventional single-cell Raman spectroscopy techniques.