Related Experiment Video For Bioimaging
Updated: Jun 11, 2025
Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
Published on: July 17, 2012
A high biocompatible near-infrared fluorescent probe for tracking cysteine in multi-biosystem and its application in
Hualong Li1, Huiling Zhang2, Zhixuan Feng2
1School of Chemistry and Chemical Engineering, Shandong University of Technology, Zibo, Shandong Province 255049, PR China; State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
Abstract:
Cysteine (Cys) plays a crucial role in the biological system and many related diseases. However, the detection of Cys in living organisms are still hindered by shortage of small molecule fluorophores that exhibit excitation and emission in the near-infrared region. Herein, we designed and synthesized a high water-soluble Cys probe (Cy7-SS) based on heptamethine cyanine scaffold. The prepared Cy7-SS displayed an enhanced emission at near-infrared region (NIR) after the recognition of Cys. Moreover, Cy7-SS not only exhibited high selectivity and sensitivity on the detection of in vitro Cys, but also could be used for endogenous Cys in living cells and C.elegans. The prepared strategy for the design of fluorophores expands the in vivo sensing toolkit for the precise analysis in clinical diagnosis.
