Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K
Homologous Recombination02:31

Homologous Recombination

50.3K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.3K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.1K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.1K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

12.5K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
12.5K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

3.1K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.1K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

3.6K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Moonlighting role of meiotic SYCP1 in breast cancer: A chromatin-bound regulator of DNA repair, transcription, and drug resistance.

Science advances·2026
Same author

Molecular architecture of meiotic pro-crossover factor HEI10 reveals coupling of higher-order assembly and ubiquitin chain formation.

bioRxiv : the preprint server for biology·2026
Same author

Correction: Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity.

eLife·2026
Same author

Synaptonemal complex assembly in yeast depends on a 2:2 Ecm11-Gmc2 heterocomplex.

Open biology·2026
Same author

SCEP3 initiates synapsis and implements crossover interference in Arabidopsis.

Nature plants·2025
Same author

Addendum: BRCA2 stabilises RAD51 and DMC1 nucleoprotein filaments through a conserved interaction mode.

Nature communications·2024

Related Experiment Video

Updated: Jun 11, 2025

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.1K

BRCA2 stabilises RAD51 and DMC1 nucleoprotein filaments through a conserved interaction mode.

James M Dunce1, Owen R Davies2

  • 1Department of Biochemistry, University of Cambridge, Cambridge, UK.

Nature Communications
|September 27, 2024
PubMed
Summary

The study reveals how BRCA2

More Related Videos

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

8.0K
Author Spotlight: Unveiling the Role of SNF2L in Replication Fork Stability and Genome Duplication
05:55

Author Spotlight: Unveiling the Role of SNF2L in Replication Fork Stability and Genome Duplication

Published on: August 23, 2024

458

Related Experiment Videos

Last Updated: Jun 11, 2025

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.1K
Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

8.0K
Author Spotlight: Unveiling the Role of SNF2L in Replication Fork Stability and Genome Duplication
05:55

Author Spotlight: Unveiling the Role of SNF2L in Replication Fork Stability and Genome Duplication

Published on: August 23, 2024

458

Area of Science:

  • Molecular Biology
  • Genetics
  • Structural Biology

Background:

  • BRCA2 protein is crucial for DNA repair via homologous recombination.
  • BRCA2 interacts with RAD51 and DMC1 recombinases to facilitate DNA repair.
  • BRCA2's PhePP motifs stabilize RAD51 and DMC1 filaments, but the mechanism was unknown.

Purpose of the Study:

  • To elucidate the structural mechanism by which BRCA2's PhePP motifs stabilize RAD51 and DMC1 nucleoprotein filaments.
  • To determine the crystal structure of a BRCA2-DMC1 complex.

Main Methods:

  • X-ray crystallography
  • Structural analysis of BRCA2-DMC1 complex

Main Results:

  • The crystal structure reveals how PhePP motifs bind to RAD51 and DMC1 recombinases.
  • The binding mode is conserved between RAD51 and DMC1, utilizing distinct binding pockets on BRCA2.
  • Surrounding sequences of PhePP motifs protect filaments from disruption.

Conclusions:

  • The study provides the structural basis for BRCA2-mediated stabilization of RAD51 and DMC1 filaments.
  • This stabilization is essential for homologous recombination in both mitosis and meiosis.
  • Understanding this mechanism is key for DNA repair processes.