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Related Experiment Video

Updated: Jun 21, 2026

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

A Simple High-Throughput Technology for Microorganism Detection and Quantitative Analysis.

Liping Wang¹, Ziyun Wu¹

¹School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200030, China.

Foods (Basel, Switzerland)

|September 28, 2024

Summary

This summary is machine-generated.

This study introduces a high-throughput microplate reader method for food safety, improving microbial detection by distinguishing viable from damaged microorganisms and reducing analysis time significantly.

Keywords:

colorimetric high throughput microbial counts microplate reader trace sample

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Area of Science:

Food microbiology
Microbial detection technologies
Food safety analysis

Background:

The colony-forming unit (CFU) assay is a traditional benchmark for microbial viability but cannot differentiate between normal and damaged microorganisms.
Accurate assessment of microbial viability is crucial for food safety and quality control.
Existing methods for microbial enumeration are often time-consuming and lack sensitivity.

Purpose of the Study:

To develop and validate an improved, high-throughput technology for quantitative microbial analysis in food.
To enable differentiation between normal and damaged microorganisms.
To provide a faster and more sensitive method for microbial detection.

Main Methods:

Utilized a visible absorbance microplate reader platform with a 96-well plate for high-throughput analysis.
Developed a method for quantitative analysis of microbial lag time, doubling time, and CFU from small sample volumes (5 microliters).
Implemented a mild temperature treatment to enhance cell viability detection and reflect true microbial quality.

Main Results:

The new platform accurately determines viable cell numbers across a dynamic range of 5–7 orders of magnitude.
Analysis time was reduced by over 20-fold compared to traditional spread plate methods.
The method demonstrated the ability to detect a single microbial cell within a well, indicating high sensitivity.

Conclusions:

The developed high-throughput microplate reader method offers significant improvements over traditional CFU assays for microbial detection in food.
This technology provides essential technical support for accurate and efficient microbial analysis, enhancing food safety.
The ability to distinguish between viable and damaged cells and detect single cells offers a more comprehensive assessment of microbial quality.