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Updated: Jun 22, 2026

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

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Published on: May 21, 2020

A Spatiotemporal Transcriptome Reveals Stalk Development in Pearl Millet.

Fei Mao¹, Lin Luo¹, Nana Ma²

¹National Engineering Research Center of JUNCAO, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology and College of Juncao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

International Journal of Molecular Sciences

|September 28, 2024

Summary

Pearl millet stalk development was studied using transcriptomes. A MYB transcription factor was identified that regulates cell wall synthesis genes, offering targets for crop improvement.

Keywords:

gene expression genetic improvement pearl millet stalk development transcriptome analysis

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Area of Science:

Plant Biology
Genomics
Agricultural Science

Background:

Pearl millet is a vital cereal crop for arid regions, feeding over 90 million people.
Stalk development is crucial for crop productivity and stress tolerance in grasses.
Molecular mechanisms of pearl millet stalk development are not well understood.

Purpose of the Study:

To elucidate the molecular mechanisms governing stalk development in pearl millet.
To identify key genes and pathways involved in stalk internode development.
To provide potential genetic targets for improving pearl millet.

Main Methods:

Spatiotemporal transcriptome analysis of 19 samples from pearl millet stalk internodes across four developmental stages.
Identification of distinct developmental zones and gene expression patterns within the stalk.
Weighted Gene Co-expression Network Analysis (WGCNA) to identify gene modules related to stalk elongation and thickening.
SELEX assay to validate transcription factor binding to target gene promoters.

Main Results:

Four distinct developmental zones were identified in pearl millet stalks.
Twelve specific gene sets with unique expression patterns were discovered.
Two co-expression modules and candidate genes were linked to stalk elongation and thickening.
A MYB transcription factor (PMF7G02448) was found to bind promoters of three CesA genes, crucial for cell wall synthesis.

Conclusions:

This study provides novel insights into the genetic regulation of pearl millet stalk development.
Identified candidate genes, including PMF7G02448, offer potential targets for enhancing pearl millet traits.
Findings contribute to the genetic improvement strategies for this important cereal crop.