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Identification of Tumor Suppressive miR-144-5p Targets: FAM111B Expression Accelerates the Malignant Phenotypes of Lung Adenocarcinoma

  • 0Department of Pulmonary Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8520, Japan.
International Journal of Molecular Sciences +

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September 28, 2024

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September 28, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

MicroRNA-144-5p (miRNA-144-5p) is downregulated in lung adenocarcinoma (LUAD). Restoring miRNA-144-5p suppresses LUAD cell aggressiveness by targeting FAM111B, offering potential therapeutic strategies.

Area Of Science

  • Molecular biology
  • Oncology
  • Genetics

Background

  • Passenger strand microRNAs (miRNAs) are implicated in cancer development.
  • microRNA-144-5p (miRNA-144-5p) is significantly downregulated in lung adenocarcinoma (LUAD) tissues.
  • This downregulation suggests a potential role in LUAD pathogenesis.

Purpose Of The Study

  • To identify therapeutic target molecules regulated by miRNA-144-5p in LUAD.
  • To investigate the function of miRNA-144-5p in controlling LUAD cell aggressiveness.
  • To explore the role of Family with Sequence Similarity 111 Member B (FAM111B) as a target of miRNA-144-5p.

Main Methods

  • miRNA expression profiling and analysis of The Cancer Genome Atlas (TCGA) data.
  • Ectopic expression assays to evaluate miRNA-144-5p function in LUAD cells.
  • In silico analysis to identify putative target genes.
  • Luciferase reporter assays and knockdown experiments to validate FAM111B regulation by miRNA-144-5p.

Main Results

  • Ectopic expression of miRNA-144-5p inhibited LUAD cell proliferation, migration, and invasion, while inducing cell cycle arrest and apoptosis.
  • Eighteen putative cancer-promoting genes were identified as targets of miRNA-144-5p.
  • FAM111B was confirmed as a direct target of miRNA-144-5p in LUAD cells.
  • FAM111B knockdown phenocopied the tumor-suppressive effects of miRNA-144-5p.

Conclusions

  • miR-144-5p acts as a tumor suppressor in LUAD by inhibiting cell proliferation, migration, and invasion.
  • FAM111B is a key oncogenic target of miR-144-5p in LUAD.
  • Targeting the miR-144-5p/FAM111B axis presents a potential therapeutic strategy for LUAD.

Keywords:

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