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Updated: Jun 11, 2025

A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium
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A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

Published on: June 22, 2010

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Circulating microRNAs in Carotid Atherosclerosis: Complex Interplay and Possible Associations with Atherothrombotic

Marine M Tanashyan1, Alla A Shabalina2, Vladislav A Annushkin1

  • 1Research Center of Neurology, 80, Volokolamskoe Shosse, 125367 Moscow, Russia.

International Journal of Molecular Sciences
|September 28, 2024
PubMed

Insights

Circulating microRNAs (miRs) show altered expression in patients with carotid atherosclerosis (CA) and a history of ischemic stroke. These miRs may serve as novel biomarkers for CA progression and stroke risk.

Area of Science:

  • Cardiovascular Research
  • Epigenetics
  • Molecular Biology

Background:

  • Atherosclerosis, particularly carotid atherosclerosis (CA), is a primary driver of cardiovascular disease and ischemic stroke.
  • Epigenetic factors, including microRNAs (miRs), are implicated in CA pathogenesis, but their specific role in stroke remains unclear.
  • Understanding circulating miR profiles in CA patients can offer insights into stroke risk and disease progression.

Purpose of the Study:

  • To investigate the association between circulating microRNA expression profiles and symptomatic carotid atherosclerosis (CA) with a history of ischemic stroke.
  • To identify specific miRs that are differentially expressed in CA patients with and without prior stroke.
  • To explore the potential of these miRs as biomarkers for CA and stroke risk.

Main Methods:

  • Plasma samples were collected from 81 patients with moderate-to-severe CA.
  • Expression levels of 24 specific microRNAs were measured using quantitative real-time PCR.
  • Statistical analyses, including multivariable logistic regression and cluster analysis, were performed to identify significant miR associations with stroke status.

Main Results:

  • Several miRs, including miR-200c-3p, miR-106b-3p, and miR-494-5p, were upregulated in stroke survivors.
  • Conversely, miR183-3p, miR-126-5p, and miR-216-3p showed lower plasma levels in symptomatic patients.
  • Multivariable analysis identified miR-106b-5p, miR-183-3p, miR-216-3p, and miR-494-5p as significant predictors of symptomatic CA.

Conclusions:

  • Circulating microRNA expression patterns differ between carotid atherosclerosis patients with and without a history of ischemic stroke.
  • Specific miRs, such as miR-106b-5p, miR-183-3p, miR-216-3p, and miR-494-5p, are potential epigenetic biomarkers for CA progression and stroke.
  • Further research into these miRs could lead to improved diagnostic and prognostic tools for cardiovascular and cerebrovascular diseases.

Keywords:
biomarkerscarotid atherosclerosiscerebrovascular diseasemicroRNAstroke

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