Related Experiment Video

Updated: May 5, 2026

A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood
06:28

A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood

Published on: August 12, 2016

16.6K

Deep Immune and RNA Profiling Revealed Distinct Circulating CD163+ Monocytes in Diabetes-Related Complications

Elisha Siwan1, Jencia Wong1,2, Belinda A Brooks2

  • 1Greg Brown Diabetes and Endocrine Research Laboratory, Sydney Medical School (Central), Faculty of Medicine and Health, Charles Perkin Centre, The University of Sydney, Sydney, NSW 2006, Australia.

International Journal of Molecular Sciences
|September 28, 2024
PubMed

Insights

Diabetes complications alter CD163+ monocytes, impacting immune regulation and cell cycle genes. These findings reveal distinct monocyte functions in diabetic patients with complications, suggesting a role in tissue damage.

Area of Science:

  • Immunology
  • Endocrinology
  • Molecular Biology

Background:

  • CD163, a scavenger receptor with anti-inflammatory properties, is expressed on monocytes/macrophages.
  • Dysregulation of CD163 is observed in diabetes complications.
  • Understanding CD163+ monocyte characteristics is crucial for diabetes research.

Purpose of the Study:

  • To characterize circulating CD163+ monocytes in adults with long-duration diabetes.
  • To compare CD163+ monocytes between diabetic patients with (D+Comps) and without (D-Comps) diabetes-related complications.

Main Methods:

  • RNA-sequencing and mass cytometry were performed on CD163+ monocytes.
  • Analysis focused on gene expression and protein markers in D+Comps versus D-Comps groups.
  • Gene-protein network analysis was utilized to identify key regulatory nodes.

Main Results:

  • 10,868 differentially expressed genes were identified between D+Comps and D-Comps.
  • Genes related to 'regulation of centrosome cycle' were significantly enriched in D+Comps.
  • CD163+ monocytes in D+Comps showed reduced expression of recruitment and immune regulation markers (e.g., CCR5, CD11b, CD39, CD86).

Conclusions:

  • CD163+ monocytes exhibit distinct functional profiles in diabetes complications.
  • Enriched centrosome cycle genes and altered miRNA expression suggest changes in cell cycle and apoptosis.
  • Down-regulated monocyte activation and recruitment markers may contribute to diabetes-related tissue damage.

Keywords:
CD163complicationsdiabetes mellitusinflammationmonocytesscavenger receptor

More Related Videos

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions09:41

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Published on: October 17, 2017

13.3K
Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis09:12

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Published on: October 17, 2018

57.6K

Related Concept Videos

Pathophysiology of Diabetes01:20

Pathophysiology of Diabetes

Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia. The four categories of diabetes are type 1 diabetes, type 2 diabetes, other specific types of diabetes, and gestational diabetes.
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...
3.9K
Diabetes: Symptoms, Diagnosis, and Complications01:15

Diabetes: Symptoms, Diagnosis, and Complications

For most patients, experiencing several weeks of polyuria, polydipsia, fatigue, and significant weight loss may indicate the presence of diabetes. Furthermore, adults displaying the phenotypic appearance of type 2 diabetes (particularly those who are obese and not initially insulin-requiring), may have islet cell autoantibodies, suggesting autoimmune-mediated β cell destruction and a diagnosis of latent autoimmune diabetes of adults (LADA). The categorization of glucose homeostasis is...
2.5K
Diabetes Mellitus: Introduction01:26

Diabetes Mellitus: Introduction

Diabetes mellitus consists of chronic metabolic disorders characterized by persistent hyperglycemia. This elevated blood glucose results from defects in insulin secretion, impaired insulin action, or both. Insulin, produced by pancreatic β-cells, is essential for maintaining glucose homeostasis by facilitating cellular glucose uptake for energy or storage. Disruptions in insulin production or function lead to glucose accumulation in the bloodstream, causing the clinical features and...
24
Type I Diabetes II: Pathophysiology01:26

Type I Diabetes II: Pathophysiology

Type 1 diabetes mellitus arises from an immune-mediated destruction of pancreatic β-cells, resulting in an absolute deficiency of insulin. This process develops in genetically susceptible individuals when autoimmunity, environmental exposures, and immunologic dysregulation converge to trigger a targeted attack on the insulin-producing cells of the pancreas. The β-cells are located within the islets of Langerhans and are essential for regulating blood glucose by facilitating cellular...
75
Type II Diabetes II: Pathophysiology01:24

Type II Diabetes II: Pathophysiology

PathophysiologyType 2 diabetes mellitus (T2DM ) is a chronic metabolic disorder characterized by insulin resistance and progressive pancreatic β-cell dysfunction, leading to impaired glucose homeostasis. It results from interactions among genetic predisposition, environmental factors, and metabolic stressors, such as overnutrition and a sedentary lifestyle.Insulin Resistance and Glucose DysregulationEarly T2DM involves insulin resistance in skeletal muscle, adipose tissue, and the liver.
28
Complications of Diabetes Mellitus01:22

Complications of Diabetes Mellitus

Diabetes mellitus is a chronic metabolic disorder characterized by persistent hyperglycemia due to insulin deficiency, resistance, or both. Prolonged hyperglycemia disrupts metabolic homeostasis and leads to acute and chronic complications.Acute ComplicationsAcute complications result from sudden metabolic imbalance.Diabetic ketoacidosis (DKA) mainly appears in type 1 diabetes but may also develop in type 2 diabetes, particularly under extreme stress. It arises from severe insulin deficiency,...
35