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Updated: Jun 11, 2025
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Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay
Published on: January 8, 2012
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Galectin-8 Contributes to Human Trophoblast Cell Invasion
Janko Legner1, Milica Jovanović Krivokuća1, Aleksandra Vilotić1
1Department for Biology of Reproduction, Institute for the Application of Nuclear Energy, University of Belgrade, 11000 Belgrade, Serbia.
International Journal of Molecular Sciences
|September 28, 2024
Summary
Galectin-8 enhances human trophoblast cell invasion, crucial for pregnancy establishment. This study reveals galectin-8
Area of Science:
- Reproductive biology
- Cellular and Molecular Biology
- Biochemistry
Background:
- Galectins are key regulators of biological processes, including development and inflammation.
- Galectin-8 is present in human placental trophoblast cells, but its function in early pregnancy is unknown.
Purpose of the Study:
- To investigate the functional role of galectin-8 in human extravillous trophoblast (EVT) cells and primary cytotrophoblasts.
- To determine galectin-8's effects on trophoblast cell adhesion, migration, and invasion.
Main Methods:
- Utilized HTR-8/SVneo EVT cell line and primary human cytotrophoblasts.
- Assessed cell adhesion, migration, and invasion.
- Analyzed integrin and matrix metalloproteinase (MMP) expression using qPCR, cELISA, and zymography.
Main Results:
- Recombinant human galectin-8 (rh galectin-8) significantly increased HTR-8/SVneo cell adhesion, migration, and invasion.
- Galectin-8 also enhanced primary cytotrophoblast invasion.
- MMP-2 and MMP-9 levels increased, while integrin alpha-1 and beta-1 expression remained unchanged.
Conclusions:
- Galectin-8 positively influences trophoblast cell invasion, suggesting a significant role in placental development.
- Galectin-8 may be a critical factor in successful pregnancy establishment through its effects on EVT invasion.
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