Synthesis and Characterization of Multifunctional Chitosan-Silver Nanoparticles: An In-Vitro Approach for Biomedical
Gulamnabi Vanti1, Naresh Poondla2,3,4, Prasath Manogaran5
1Multidisciplinary Research Unit, Karnataka Medical College and Research Institute, Hubli 580021, India.
Pharmaceuticals (Basel, Switzerland)
|September 28, 2024
Summary
Novel silver chitosan nanoparticles combat multi-drug-resistant bacteria and promote healing. These non-toxic nanoparticles show antibacterial, antioxidant, and wound healing properties for biomedical applications.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Infectious Diseases
Background:
- Antibiotics are crucial for public health but their overuse has led to multi-drug-resistant bacteria.
- Novel antimicrobial agents with broad therapeutic potential are urgently needed.
- Silver chitosan nanoparticles offer a promising alternative due to their versatile properties.
Purpose of the Study:
- To synthesize and characterize silver chitosan nanoparticles (SCNPs).
- To evaluate the antibacterial, non-toxic, wound healing, and antioxidant potential of SCNPs.
- To explore the biomedical application compatibility of SCNPs.
Main Methods:
- Synthesis and characterization of SCNPs using FT-IR, XRD, and size analysis.
- Antibacterial activity testing against *S. aureus* and *E. coli*.
- MTT assay for cytotoxicity, scratch assay for wound healing, and DCFH-DA staining for antioxidant activity.
Main Results:
- SCNPs were synthesized with sizes of 14-25 nm and a positive surface charge.
- SCNPs demonstrated significant antibacterial activity against *S. aureus* and *E. coli*.
- SCNPs exhibited low toxicity, promising wound healing capabilities, and notable antioxidant properties.
Conclusions:
- Synthesized SCNPs possess a versatile profile suitable for biomedical applications.
- SCNPs present a viable option for combating antibiotic resistance and aiding tissue repair.
- Further research into SCNPs could lead to advanced therapeutic strategies.
