Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Rab Proteins

Rab Proteins

Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Bovine Lactoferrin Inhibits Pseudorabies Virus Attachment Through Blockade of Glycoprotein C-Heparan Sulfate Interaction.

Transboundary and emerging diseases·2026

Same author

Establishment and Application of an Indirect ELISA for Detecting Getah Virus IgG Antibodies in Swine Based on the E2EP3 Peptide.

Veterinary sciences·2026

Same author

Rab4b is associated with inflammatory responses and TLR3-related signaling during Japanese encephalitis virus infection.

Virology·2026

Same author

25-Hydroxycholesterol Restricts Japanese Encephalitis Virus via Metabolic Suppression of the SREBP2-Mediated Signaling Axis.

Microorganisms·2026

Same author

The Host NADase CD38 Promotes JEV Replication by Targeting the NAD+/SIRT1 Axis.

Microorganisms·2026

Same author

CD4 Molecule Plays an Important Role in the Inflammatory Response Induced by Japanese Encephalitis Virus Infection.

Veterinary sciences·2026

Search research articles

Related Experiment Video

Updated: Jun 11, 2025

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

Host Factor Rab4b Promotes Japanese Encephalitis Virus Replication.

Qin Zhao^1,2,3, Chang Miao¹, Yi-Ting Chen¹

¹Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

|September 28, 2024

Summary

This summary is machine-generated.

Rab4b protein is crucial for Japanese encephalitis virus (JEV) replication. Knocking out Rab4b inhibits JEV, while overexpressing it promotes viral proliferation, impacting adsorption, internalization, assembly, and release.

Keywords:

Japanese encephalitis virus Rab4b host factor viral replication

More Related Videos

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line

Published on: February 17, 2016

Related Experiment Videos

Last Updated: Jun 11, 2025

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line

Published on: February 17, 2016

Area of Science:

Virology
Cell Biology
Molecular Biology

Background:

Japanese encephalitis virus (JEV) infects various cell types, but its host receptors remain unclear.
Previous studies identified Rab4b as a potential host factor for JEV infection in PK15 cells using CRISPR/Cas9 screening.

Purpose of the Study:

To investigate the role of Rab4b in the JEV life cycle.
To elucidate how Rab4b influences JEV replication in PK15 cells.

Main Methods:

Utilized CRISPR/Cas9 technology to create Rab4b knockout and overexpression PK15 cell lines.
Employed immunofluorescence assay (IFA), RT-qPCR, and Western blot to analyze JEV replication.
Studied the impact of Rab4b on JEV adsorption, internalization, assembly, and release.

Main Results:

Rab4b knockout significantly inhibited JEV replication in PK15 cells.
Rab4b overexpression promoted JEV replication in PK15 cell lines.
Demonstrated Rab4b's role in facilitating JEV adsorption, internalization, assembly, and release.

Conclusions:

Host factor Rab4b plays a critical role in promoting JEV replication throughout its entire life cycle.
This study enhances understanding of the JEV-host factor interaction network.
Provides a foundation for further research into Rab4b protein function in viral infections.